Chip-Scale Universal Detection Based on Backscatter Interferometry

• Published in: Analytical chemistry (Washington) 2000-07, Vol.72 (13), p.2690-2695
• Main Authors: Swinney, Kelly, Markov, Dmitry, Bornhop, Darryl J
• Format: Article
• Language: English
• Subjects: Biochemistry; Chemistry; Exact sciences and technology; Instruments, apparatus, components and techniques common to several branches of physics and astronomy; Interferometers; Optical instruments, equipment and techniques; Physics
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac000261r

An on-chip detector based on backscatter interferometry has been developed to perform subnanoliter-volume refractive index measurements. The detection system consists of a simple, folded optical train based on the interaction of a laser beam and an etched channel, consisting of two radii joined by a flat portion, thus defining a curved surface in the shape of a hemisphere in a silica (glass) plate. The backscattered light from the channel takes on the form of a high-contrast interference pattern that contains information related to the bulk properties of the fluid contained within the probe volume. Positional changes of the interference pattern (fringes) allow for the determination of Δn at the 10-6 level, corresponding to 743 μM or 139 × 10-15 mol or 12.8 × 10-12 g of sucrose, in a probe volume of only 188 × 10-12 L. A theoretical model of the on-chip backscatter interferometric detector has also been developed, evaluated, and found to be in agreement with experimental data. It is shown that the model can be used to predict general system performance for changes in the optical train such as the chip's wall thickness and channel diameter.