Force Modulation Elasticity Mapping of Plastic-embedded, Thin-sectioned Skeletal Muscle

We have been researching the capability of atomic force microscopy to reveal nontopographic properties of tissue embedded in plastic and sectioned with standard electron microscopic techniques. We present topography and elasticity maps of plastic-embedded, thin sections of muscle tissue. The images...

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Bibliographic Details
Published in:Microscopy and microanalysis 2001-01, Vol.7 (1), p.32-38
Main Authors: Akhremitchev, Boris B., Brown, Henry G., Graner, Scott R., Walker, Gilbert C.
Format: Article
Language:English
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Summary:We have been researching the capability of atomic force microscopy to reveal nontopographic properties of tissue embedded in plastic and sectioned with standard electron microscopic techniques. We present topography and elasticity maps of plastic-embedded, thin sections of muscle tissue. The images show topography correlated with the normal repeating structure of the sarcomere. Elasticity mapping using force modulation revealed contrast between the actin- and myosin-rich areas. We attribute the observed contrast in elasticity to the difference in local concentrations of biological material in embedding plastic.
ISSN:1431-9276
1435-8115
DOI:10.1007/S100050010055