Effects of cytokinin on ethylene production and nodulation in pea (Pisum sativum) cv. Sparkle

In this study, we were interested in learning if cytokinins play a role in the developmental process that leads to nodulation in the pea cv. Sparkle. We demonstrate that the application of the synthetic cytokinin BAP (6‐benzyl‐amino‐purine) results in a number of nodulation‐related changes. BAP stim...

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Bibliographic Details
Published in:Physiologia plantarum 2001-07, Vol.112 (3), p.421-428
Main Authors: Lorteau, M.-A, Ferguson, B.J, Guinel, F.C. (Waterloo Univ., Ontario (Canada))
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
CITOQUININAS
CYTOKININE
CYTOKININS
Economic plant physiology
ETHYLENE
ETILENO
FORMATION DE NODOSITES
Fundamental and applied biological sciences. Psychology
NODULACION
Parasitism and symbiosis
PISUM SATIVUM
Plant physiology and development
ROOT NODULATION
Symbiosis
Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
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Summary:In this study, we were interested in learning if cytokinins play a role in the developmental process that leads to nodulation in the pea cv. Sparkle. We demonstrate that the application of the synthetic cytokinin BAP (6‐benzyl‐amino‐purine) results in a number of nodulation‐related changes. BAP stimulates the production of ethylene, a known inhibitor of nodulation. At low levels (up to 1 μM), BAP also stimulates nodulation but as its concentration is increased (up to 25 μM), nodule number decreases. In BAP‐treated roots, the infection threads are abnormal; they are twisted, very knotty, and generally grow in a direction parallel to the root surface. In addition, the centers of cell division in the inner cortex are very few. Thus, BAP‐treated Sparkle appears to phenocopy the low‐nodulating pea mutant R50 [Guinel FC, Sloetjes LL (2000) Ethylene is involved in the nodulation phenotype of Pisum sativum R50 (sym 16), a pleiotropic mutant that nodulates poorly and has pale green leaves. J Exp Bot 51: 885–894]. However, it appears doubtful that there is a direct correlation between the actions of cytokinin and ethylene in causing a reduction in nodule organogenesis because nodulation is not restored by treating BAP‐treated Sparkle with ethylene inhibitors.
ISSN:0031-9317
1399-3054
DOI:10.1034/j.1399-3054.2001.1120316.x