A male and hermaphrodite specific RAPD marker for papaya (Carica papaya L.)

The random amplified polymorphic DNA (RAPD) technique was used to determine the sex of a dioecious species, Carica papayaL., with three sex types, male, female and hermaphrodite. A 450 bp marker fragment, named PSDM(Papaya Sex Determination Marker), exists in all male and hermaphrodite plants but no...

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Bibliographic Details
Published in:Theoretical and applied genetics 2002-02, Vol.104 (2-3), p.281-285
Main Authors: URASAKI, N, TOKUMOTO, M, TARORA, K, BAN, Y, KAYANO, T, TANAKA, H, OKU, H, CHINEN, I, TERAUCHI, R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carica papaya
Classical genetics, quantitative genetics, hybrids
Cloning
DNA polymerase
Fruits
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Pteridophyta, spermatophyta
Vegetals
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Summary:The random amplified polymorphic DNA (RAPD) technique was used to determine the sex of a dioecious species, Carica papayaL., with three sex types, male, female and hermaphrodite. A 450 bp marker fragment, named PSDM(Papaya Sex Determination Marker), exists in all male and hermaphrodite plants but not in the female plants so far analyzed. The DNA sequence of PSDM exhibited no significant similarity to previously reported sequences. A sequence-characterized amplified region (SCAR) marker, SCARps, was developed from PSDM to determine the sex of papaya. Southern hybridization, using PSDM as a probe, showed that PSDM exists in the male and hermaphrodite genomes, but not in the female genome. This result strongly suggests that PSDM is located on the chromosome region that is specific to the male and the hermaphrodite. SCARps is a suitable marker for the precise and rapid diagnosis of sex in papaya.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220100693