Cre/lox site-specific recombination controls the excision of a transgene from the rice genome

The Cre/ lox site-specific recombination controls the excision of a target DNA segment by recombination between two loxsites flanking it, mediated by the Cre recombinase. We have studied the functional expression of the Cre/ lox system to excise a transgene from the rice genome. We developed transge...

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Bibliographic Details
Published in:Theoretical and applied genetics 2002-03, Vol.104 (4), p.518-525
Main Authors: HOA, T. T. C, BONG, B. B, HUQ, E, HODGES, T. K
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Classical genetics, quantitative genetics, hybrids
Cloning
Embryos
Fundamental and applied biological sciences. Psychology
Genetic aspects
Genetic engineering
Genetic technics
Genetics of eukaryotes. Biological and molecular evolution
Genomes
Genomics
Methods. Procedures. Technologies
Physiological aspects
Plant genetics
Plasmids
Pteridophyta, spermatophyta
Rice
Transgenic animals and transgenic plants
Transgenic plants
Vegetals
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Summary:The Cre/ lox site-specific recombination controls the excision of a target DNA segment by recombination between two loxsites flanking it, mediated by the Cre recombinase. We have studied the functional expression of the Cre/ lox system to excise a transgene from the rice genome. We developed transgenic plants carrying the target gene, hygromycin phosphotransferase ( hpt) flanked by two lox sites and transgenic plants harboring the Cre gene. Each lox plant was crossed with each Cre plant reciprocally. In the Cre /lox hybrid plants, the Cre recombinase mediates recombination between two lox sites, resulting in excision of the hpt gene. The recombination event could be detected because it places the CaMV 35S promoter of the hpt gene adjacent to a promoterless gusA gene; as a result the gusA gene is activated and its expression could be visualized. In 73 Cre /lox hybrid plants from various crosses of T0 transgenic plants, 19 expressed GUS, and in 132 Cre /lox hybrid plants from crosses of T2 transgenic plants, 77 showed GUS expression. Molecular data proved the excision event occurred in all the GUS(+) plants. Recombination occurred with high efficiency at the early germinal stage, or randomly during somatic development stages.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220100748