Identification of an allele of CLA1 associated with variegation in Arabidopsis thaliana

We have identified a mutant of Arabidopsis thaliana (lvr111) that exhibits a variegated phenotype, reduced isoprenoid pigmentation, and dwarfism in comparison with wild‐type plants. Segregation analysis indicated that this phenotype was caused by a single, semi‐dominant mutation and PCR‐based marker...

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Bibliographic Details
Published in:Physiologia plantarum 2003-05, Vol.118 (1), p.29-37
Main Authors: Crowell, Dring N., Packard, Clay E., Pierson, Charles A., Giner, José-Luis, Downes, Brian P., Chary, S. Narasimha
Format: Article
Language:English
Subjects:
Arabidopsis thaliana
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Metabolism
Metabolism. Physicochemical requirements
Plant physiology and development
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Summary:We have identified a mutant of Arabidopsis thaliana (lvr111) that exhibits a variegated phenotype, reduced isoprenoid pigmentation, and dwarfism in comparison with wild‐type plants. Segregation analysis indicated that this phenotype was caused by a single, semi‐dominant mutation and PCR‐based marker mapping placed the mutation near position 56 on the RI map of chromosome IV. The lvr111 lesion was identified by genomic PCR and sequence analysis as a missense mutation (D306N) in the CLA1 gene (AT4g15560) and complementation analysis confirmed the allelic relationship between lvr111 and CLA1. CLA1 encodes 1‐deoxy‐d‐xylulose 5‐phosphate synthase, which catalyses the first step of the non‐mevalonate isoprenoid biosynthetic pathway. These observations demonstrate that, unlike the albinism caused by severe alleles of CLA1, weaker alleles are associated with leaf variegation.
ISSN:0031-9317
1399-3054
DOI:10.1034/j.1399-3054.2003.00063.x