Sensitivity of the mosquito Aedes aegypti (Culicidae) labral apical chemoreceptors to blood plasma components

• Published in: Journal of insect physiology 1999-05, Vol.45 (5), p.485-491
• Main Authors: Werner-Reiss, Uri, Galun, Rachael, Crnjar, Roberto, Liscia, Anna
• Format: Article
• Language: English
• Subjects: Aedes aegypti; Blood plasma components; Culicidae; Electrophysiology; Freshwater; Labral apical chemoreceptors; Tip-recording
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/S0022-1910(98)00151-6

The phagostimulants from the cellular fraction of blood induce gorging of Aedes aegypti (L.), and this process is enhanced by some plasma components. This project examines the responses of the labral apical chemoreceptors to plasma components enhancing phagostimulation. From the electrophysiological responses of the labral apical chemoreceptors four cells were identified by the waveform of their action potentials. Three of the cells (Cell 2, Cell 3 and Cell 4) responded in a dose dependent manner to NaCl. The responses of Cell 2 and Cell 3 to NaCl concentrations from 1 to 500 mmol/l can be described by a logarithmic equation. The response of Cell 2 to 150 mmol/l NaCl is modulated when a buffer is added. The magnitude of the modulation of the response is determined by the nature of the buffer: NaHCO 3 inhibits while Na 2HPO 4 enhances the response. High osmotic pressure inhibits the response of Cell 4, regardless of how it is achieved. Cell 4 responds with a high frequency to the presence of l-alanine, the C-terminal amino acid of albumin, but shows a reduced response to the same concentration of albumin. From these results it can be concluded that labral apical chemoreceptors of A. aegypti are capable of detecting the plasma components involved in blood recognition.