In vitro regeneration of plantlets from mature embryos of Pinus ayacahuite

A plantlet regeneration protocol was developed for Pinus ayacahuite var. ayacahuite (Ehrenb.). Embryos from mature seeds from ten provenances were cultured in a 16-h photoperiod for 3 days on a medium containing 30 mM sucrose and 0.7% agar. Cotyledons from these embryos were subcultured onto MCM med...

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Bibliographic Details
Published in:Tree physiology 1997-12, Vol.17 (12), p.787-796
Main Authors: Saborio, F, Dvorak, W.S, Donahue, J.K, Thorpe, T.A
Format: Article
Language:English
Subjects:
buds
culture media
dose response
embryo (plant)
explants
genetic variation
genotype
growth
in vitro culture
methodology
micropropagation
organogenesis
Pinus ayacahuite
plant growth substances
provenance
regenerative ability
roots
shoots
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Summary:A plantlet regeneration protocol was developed for Pinus ayacahuite var. ayacahuite (Ehrenb.). Embryos from mature seeds from ten provenances were cultured in a 16-h photoperiod for 3 days on a medium containing 30 mM sucrose and 0.7% agar. Cotyledons from these embryos were subcultured onto MCM medium (Bornman 1983) supplemented with 50 micromolar N(6)-benzyladenine and 90 mM sucrose for 2 weeks. Bud development and shoot elongation were maximized by subculturing the explants on half strength AE medium (von Arnold and Ericksson 1981), supplemented with 60 mM sucrose and 0.05% activated charcoal every 30 days. Seed source had a significant effect on the responses of the embryos to the bud induction protocol. For the provenance with the best response to bud induction, about 79% of the cultured cotyledons formed buds, and each cotyledon formed a mean of 9.1 buds, so that about 70 shoots could be induced from each seed. The best rooting response (40% rooting) was obtained by treating the shoots for 8 h with 100 micromolar naphthalene acetic acid.
ISSN:0829-318X
1758-4469
DOI:10.1093/treephys/17.12.787