Survival of Escherichia coli O157:H7 and Salmonella Muenchen on apples as affected by application of commercial fruit waxes

• Published in: International journal of food microbiology 2002-08, Vol.77 (3), p.223-231
• Main Authors: Kenney, Stephen J, Beuchat, Larry R
• Format: Article
• Language: English
• Subjects: Apple; Biological and medical sciences; Colony Count, Microbial; Escherichia coli O157 - drug effects; Escherichia coli O157 - growth & development; Escherichia coli O157:H7; Food industries; Food Microbiology; Food Preservation - methods; Fruit and vegetable industries; Fruit wax; Fundamental and applied biological sciences. Psychology; Malus - microbiology; Salmonella; Salmonella - drug effects; Salmonella - growth & development; Temperature; Time Factors; Waxes - pharmacology
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/S0168-1605(02)00113-7

Survival of Escherichia coli O157:H7, Salmonella Muenchen, and yeasts and molds on apples as affected by application of five commercial apple waxes was investigated. Red Delicious cv. apples at 21 °C were spot inoculated with E. coli O157:H7 and S. Muenchen and spray coated with waxes. Apples sprayed with water served as controls. Apples were dried at either 21 or 55 °C for 2 min before subjecting to microbiological analysis after storage for 0, 1, 3, 6, and 12 weeks at 2 or 21 °C. Drying temperature did not significantly influence populations of E. coli O157:H7 and S. Muenchen. Waxing reduced populations E. coli O157:H7 and S. Muenchen by up to 1.48 log 10 cfu/apple. Compared to untreated apples, treatment of apples with water or waxes resulted in significant ( P≤0.05) reductions in populations of E. coli O157:H7 and S. Muenchen during storage at 2 °C. Reductions on waxed apples stored at 21 °C were not as marked compared to reductions on waxed apples stored at 2 °C. With the exception of one wax, drying temperature did not significantly influence populations of yeasts and molds. Mold populations were less affected by wax applications than were yeasts, and were detected in higher numbers on apples treated with three of the five waxes compared to populations recovered from untreated control apples. None of the waxes evaluated can be relied upon to kill or remove E. coli O157:H7 and Salmonella on apples.