RNA interference of three up-regulated transcripts associated with insecticide resistance in an imidacloprid resistant population of Leptinotarsa decemlineata

• Published in: Pesticide biochemistry and physiology 2017-01, Vol.135, p.35-40
• Main Authors: Clements, Justin, Schoville, Sean, Peterson, Nathan, Huseth, Anders S., Lan, Que, Groves, Russell L.
• Format: Article
• Language: English
• Subjects: Animals; Coleoptera - drug effects; Coleoptera - genetics; Colorado potato beetle; Cytochrome P-450 Enzyme System - genetics; Cytochrome p450; Glutathione Synthase - genetics; Imidacloprid; Imidazoles - toxicity; Insect Proteins - genetics; Insecticide Resistance - genetics; Insecticides - toxicity; Leptinotarsa decemlineata; Neonicotinoids; Nitro Compounds - toxicity; Pest management; RNA Interference; Solanum tuberosum; Transcription, Genetic; Up-Regulation
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2016.07.001

The Colorado potato beetle, Leptinotarsa decemlineata (Say), is a major agricultural pest of potatoes in the Central Sands production region of Wisconsin. Previous studies have shown that populations of L. decemlineata have become resistant to many classes of insecticides, including the neonicotinoid insecticide, imidacloprid. Furthermore, L. decemlineata has multiple mechanisms of resistance to deal with a pesticide insult, including enhanced metabolic detoxification by cytochrome p450s and glutathione S-transferases. With recent advances in the transcriptomic analysis of imidacloprid susceptible and resistant L. decemlineata populations, it is possible to investigate the role of candidate genes involved in imidacloprid resistance. A recently annotated transcriptome analysis of L. decemlineata was obtained from select populations of L. decemlineata collected in the Central Sands potato production region, which revealed a subset of mRNA transcripts constitutively up-regulated in resistant populations. We hypothesize that a portion of the up-regulated transcripts encoding for genes within the resistant populations also encode for pesticide resistance and can be suppressed to re-establish a susceptible phenotype. In this study, a discrete set of three up-regulated targets were selected for RNA interference experiments using a resistant L. decemlineata population. Following the successful suppression of transcripts encoding for a cytochrome p450, a cuticular protein, and a glutathione synthetase protein in a select L. decemlineata population, we observed reductions in measured resistance to imidacloprid that strongly suggest these genes control essential steps in imidacloprid metabolism in these field populations. [Display omitted] •Studied an imidacloprid resistant field population of Leptinotarsa decemlineata for mechanisms of insecticide resistance.•RNA interference suppressed transcripts which encode for mechanisms of resistance in an imidacloprid resistant population.•Suppression of transcripts encoding for mechanisms of insecticide resistance resulted in a trend of increased susceptibility.