Practical method combining loop-mediated isothermal amplification and bait trap to detect Pythium helicoides from hydroponic culture solutions

Two detection methods combining loop-mediated isothermal amplification (LAMP) and a bait trap were developed to detect Pythium helicoides in greenhouses containing roses, miniature roses, and poinsettias in hydroponic culture systems. In “Bait-LAMP”, a crude extract derived from perilla seeds as the...

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Bibliographic Details
Published in:Journal of general plant pathology : JGPP 2017-01, Vol.83 (1), p.1-6
Main Authors: Miyake, Noriyuki, Nagai, Hirofumi, Kato, Shinro, Matsusaki, Masashi, Fukuta, Shiro, Takahashi, Reiko, Suzuki, Ryoji, Ishiguro, Yasushi
Format: Article
Language:English
Subjects:
Agriculture
Baits
Biomedical and Life Sciences
Cell culture
Extraction processes
Hydroponics
Life Sciences
Microbiology
Perilla
Plant Pathology
Pythium
Technique
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Summary:Two detection methods combining loop-mediated isothermal amplification (LAMP) and a bait trap were developed to detect Pythium helicoides in greenhouses containing roses, miniature roses, and poinsettias in hydroponic culture systems. In “Bait-LAMP”, a crude extract derived from perilla seeds as the bait was used in the LAMP reaction, whereas in the “Bait culture-LAMP”, a crude extract of mycelia grown out from perilla seeds onto Pythium -selective medium served as the bait. The two methods are simple and rapid for practical monitoring of P . helicoides in hydroponic culture systems.
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-016-0691-6