Cu/Zn superoxide dismutase (SOD) and catalase (CAT) response to crude oil exposure in the polychaete Perinereis aibuhitensis

• Published in: Environmental science and pollution research international 2017, Vol.24 (1), p.616-627
• Main Authors: Zhao, Huan, Li, Wanjuan, Zhao, Xinda, Li, Xu, Yang, Dazuo, Ren, Hongwei, Zhou, Yibing
• Format: Article
• Language: English
• Subjects: Amino acids; Animals; Antioxidants; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Biomarkers; Catalase - genetics; Catalase - metabolism; Cloning; Crude oil; DNA, Complementary - genetics; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Enzymatic activity; Enzymes; Exposure; Gene expression; Gene Expression Regulation, Enzymologic - drug effects; Genes; Heavy metals; Hydrocarbons; Laboratories; Metabolism; Metabolites; Oil pollution; Perinereis aibuhitensis; Petroleum - toxicity; Pollutants; Pollution; Polychaeta - drug effects; Polychaeta - enzymology; Polychaeta - genetics; Research Article; Sediments; Soil contamination; Studies; Superoxide Dismutase-1 - genetics; Superoxide Dismutase-1 - metabolism; Toxicity; Waste Water Technology; Water Management; Water Pollutants, Chemical - toxicity; Water Pollution Control; Worms; Zinc
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-016-7594-0

Cu/Zn superoxide dismutase (SOD) and catalase (CAT) cDNAs from the polychaete Perinereis aibuhitensis were cloned and characterized in order to investigate the relationship between crude oil exposure and stress response in this worm. The full length of PaSOD was 870 bp and PaCAT was 1967 bp encoding 150 and 506 amino acids, respectively. Gene expression and enzyme activity of Cu/Zn SOD and CAT in response to crude oil contaminated soil (500, 1500, and 3000 mg/kg) were measured. The results showed that expression of the CAT gene and enzyme activity in P. aibuhitensis was positively correlated to the concentration of crude oil and reached a maximum at 15 days of exposure to 3000 mg/kg crude oil. The expression of the SOD gene and enzyme activity of SOD in P. aibuhitensis also increased during exposure to crude oil and reached a maximum at 10 days of exposure to 3000 mg/kg crude oil. These results indicated that SOD and CAT are important for maintaining the balance of cellular metabolism and protecting P. aibuhitensis from crude oil toxicity.