Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged alpha 2-8-sialosides and high-throughput substrate specificity studies of alpha 2-8-sialidases

para-Nitrophenol (pNP)-tagged alpha 2-8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme alpha 2-8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the alpha 2-8-si...

Full description

Saved in:
Bibliographic Details
Published in:Organic & biomolecular chemistry 2017-01, Vol.15 (1), p.160-167
Main Authors: Tasnima, Nova, Yu, Hai, Li, Yanhong, Santra, Abhishek, Chen, Xi
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:para-Nitrophenol (pNP)-tagged alpha 2-8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme alpha 2-8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the alpha 2-8-sialidase activity of a recombinant human cytosolic sialidase hNEU2 and various bacterial sialidases. The sialoside substrate profiles obtained can be used to guide the selection of suitable sialidases for sialylglycan analysis and for cell and tissue surface glycan modification. They can also be used to guide sialidase inhibitor design.
ISSN:1477-0520
1477-0539
DOI:10.1039/c6ob02240e