Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged alpha 2-8-sialosides and high-throughput substrate specificity studies of alpha 2-8-sialidases

para-Nitrophenol (pNP)-tagged alpha 2-8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme alpha 2-8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the alpha 2-8-si...

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Published in:Organic & biomolecular chemistry 2017-01, Vol.15 (1), p.160-167
Main Authors: Tasnima, Nova, Yu, Hai, Li, Yanhong, Santra, Abhishek, Chen, Xi
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creator Tasnima, Nova
Yu, Hai
Li, Yanhong
Santra, Abhishek
Chen, Xi
description para-Nitrophenol (pNP)-tagged alpha 2-8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme alpha 2-8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the alpha 2-8-sialidase activity of a recombinant human cytosolic sialidase hNEU2 and various bacterial sialidases. The sialoside substrate profiles obtained can be used to guide the selection of suitable sialidases for sialylglycan analysis and for cell and tissue surface glycan modification. They can also be used to guide sialidase inhibitor design.
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title Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged alpha 2-8-sialosides and high-throughput substrate specificity studies of alpha 2-8-sialidases
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