Structural basis for cholinergic regulation of neural circuits in the mouse olfactory bulb

• Published in: Journal of comparative neurology (1911) 2017-02, Vol.525 (3), p.574-591
• Main Authors: Hamamoto, Masakazu, Kiyokage, Emi, Sohn, Jaerin, Hioki, Hiroyuki, Harada, Tamotsu, Toida, Kazunori
• Format: Article
• Language: English
• Subjects: AB_11180610; AB_2079760; AB_2187708; AB_2301794; AB_2315522; AB_572263; AB_887872; AB_94952; acetylcholine; Animals; centrifugal projection; Choline O-Acetyltransferase - genetics; Choline O-Acetyltransferase - metabolism; cholinergic neuron; Cholinergic Neurons - cytology; Cholinergic Neurons - metabolism; Dependovirus; Electron Microscope Tomography; Genetic Vectors; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Imaging, Three-Dimensional; Immunohistochemistry; IMSR_JAX:006410; Male; Mice, Inbred C3H; Mice, Transgenic; Neuroanatomical Tract-Tracing Techniques; nif‐0000–30467; olfactory bulb; Olfactory Bulb - cytology; Olfactory Bulb - metabolism; Olfactory Pathways - cytology; Olfactory Pathways - metabolism; RRIDs: SCR_001775; Smell - physiology; synapse
• ISSN: 0021-9967; 1096-9861
• DOI: 10.1002/cne.24088

ABSTRACT Odor information is regulated by olfactory inputs, bulbar interneurons, and centrifugal inputs in the olfactory bulb (OB). Cholinergic neurons projecting from the nucleus of the horizontal limb of the diagonal band of Broca and the magnocellular preoptic nucleus are one of the primary centrifugal inputs to the OB. In this study, we focused on cholinergic regulation of the OB and analyzed neural morphology with a particular emphasis on the projection pathways of cholinergic neurons. Single‐cell imaging of a specific neuron within dense fibers is critical to evaluate the structure and function of the neural circuits. We labeled cholinergic neurons by infection with virus vector and then reconstructed them three‐dimensionally. We also examined the ultramicrostructure of synapses by electron microscopy tomography. To further clarify the function of cholinergic neurons, we performed confocal laser scanning microscopy to investigate whether other neurotransmitters are present within cholinergic axons in the OB. Our results showed the first visualization of complete cholinergic neurons, including axons projecting to the OB, and also revealed frequent axonal branching within the OB where it innervated multiple glomeruli in different areas. Furthermore, electron tomography demonstrated that cholinergic axons formed asymmetrical synapses with a morphological variety of thicknesses of the postsynaptic density. Although we have not yet detected the presence of other neurotransmitters, the range of synaptic morphology suggests multiple modes of transmission. The present study elucidates the ways that cholinergic neurons could contribute to the elaborate mechanisms involved in olfactory processing in the OB. J. Comp. Neurol. 525:574–591, 2017. © 2016 Wiley Periodicals, Inc. We demonstrated visualization and reconstruction of cholinergic neurons projecting to the olfactory bulb (OB) by infection with virus vector into the horizontal limb of the diagonal band of Broca and magnocellular preoptic nuclei of ChAT‐Cre mice. Cholinergic axons exhibited multiple branchings and distributed to multiple glomeruli in the glomerular layer of OB.