Influence of Onabotulinumtoxin A on testes of the growing rat

Onabotulinumtoxin A (onabotA) is gaining wide medical use in children. The present study was planned to investigate the influence of its injection on the maturing testicular structures in rats. Immature rats were injected in the bilateral cremaster muscles by onabotA with three doses of (10, 20, and...

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Bibliographic Details
Published in:Journal of biochemical and molecular toxicology 2016-12, Vol.30 (12), p.608-613
Main Authors: Breikaa, Randa M., Mosli, Hisham A., Abdel-Naim, Ashraf B.
Format: Article
Language:English
Subjects:
Animals
apoptosis
Apoptosis - drug effects
Apoptosis - genetics
bcl-2-Associated X Protein - genetics
bcl-2-Associated X Protein - metabolism
Botulinum Toxins, Type A - pharmacology
Caspase 3 - genetics
Caspase 3 - metabolism
Cell Survival - drug effects
fas Receptor - genetics
fas Receptor - metabolism
Gene Expression Regulation - drug effects
Injections, Intramuscular
Male
Onabotulinumtoxin A. rats
Ploidies
Rats
Rats, Sprague-Dawley
Semen Analysis
Sexual Maturation - genetics
Sperm Motility - drug effects
spermatogenesis
Spermatozoa - cytology
Spermatozoa - drug effects
Spermatozoa - metabolism
testes
Testis - cytology
Testis - drug effects
Testis - metabolism
Testosterone - blood
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Summary:Onabotulinumtoxin A (onabotA) is gaining wide medical use in children. The present study was planned to investigate the influence of its injection on the maturing testicular structures in rats. Immature rats were injected in the bilateral cremaster muscles by onabotA with three doses of (10, 20, and 40 U/kg) three times in a 2‐week interval. The effect of these injections on fertility indices was examined. Levels of antisperm antibodies and several apoptosis parameters were also investigated. DNA content in form of ploidy and histopathological alterations were assessed. OnabotA‐injected groups showed decreased sperm count and semen quality, while sperm vitality, morphology, and testosterone levels were not significantly affected. Furthermore, DNA flow cytometric analysis confirmed delayed sperm maturation. Apoptosis markers were significantly increased by the injections. In conclusion, onabotA injection in growing rats adversely affected sperm count and maturation. OnabotA testicular effects are mediated, at least partly, by apoptosis.
ISSN:1095-6670
1099-0461
DOI:10.1002/jbt.21828