Mapping of stripe rust resistance gene in an Aegilops caudata introgression line in wheat and its genetic association with leaf rust resistance

• Published in: Journal of genetics 2016-12, Vol.95 (4), p.933-938
• Main Authors: TOOR, PUNEET INDER, KAUR, SATINDER, BANSAL, MITALY, YADAV, BHARAT, CHHUNEJA, PARVEEN
• Format: Article
• Language: English
• Subjects: Aegilops caudata; Amphibians; Animal Genetics and Genomics; Biomedical and Life Sciences; Caudata; Chromosomes; Evolutionary Biology; Flowers & plants; Genes; Genetics; Genomes; Genomics; Life Sciences; Microbial Genetics and Genomics; Plant genetics; Plant Genetics and Genomics; Research Article; Resistance to control; Triticum aestivum; Wheat
• ISSN: 0022-1333; 0973-7731
• DOI: 10.1007/s12041-016-0718-y

A pair of stripe rust and leaf rust resistance genes was introgressed from Aegilops caudata , a nonprogenitor diploid species with the CC genome, to cultivated wheat. Inheritance and genetic mapping of stripe rust resistance gene in backcross-recombinant inbred line (BC-RIL) population derived from the cross of a wheat– Ae. caudata introgression line (IL) T291-2(pau16060) with wheat cv. PBW343 is reported here. Segregation of BC-RILs for stripe rust resistance depicted a single major gene conditioning adult plant resistance (APR) with stripe rust reaction varying from TR-20MS in resistant RILs signifying the presence of some minor genes as well. Genetic association with leaf rust resistance revealed that two genes are located at a recombination distance of 13%. IL T291-2 had earlier been reported to carry introgressions on wheat chromosomes 2D, 3D, 4D, 5D, 6D and 7D. Genetic mapping indicated the introgression of stripe rust resistance gene on wheat chromosome 5DS in the region carrying leaf rust resistance gene LrAc , but as an independent introgression. Simple sequence repeat (SSR) and sequence-tagged site (STS) markers designed from the survey sequence data of 5DS enriched the target region harbouring stripe and leaf rust resistance genes. Stripe rust resistance locus, temporarily designated as YrAc , mapped at the distal most end of 5DS linked with a group of four colocated SSRs and two resistance gene analogue (RGA)-STS markers at a distance of 5.3 cM. LrAc mapped at a distance of 9.0 cM from the YrAc and at 2.8 cM from RGA-STS marker Ta5DS_2737450 , YrAc and LrAc appear to be the candidate genes for marker-assisted enrichment of the wheat gene pool for rust resistance.