Metabolic profiles of the Flos Abelmoschus manihot extract by intestinal bacteria from the normal and CKD model rats based on UPLC‐Q‐TOF/MS

Flos Abelmoschus manihot is a traditional herbal medicine widely used in clinical practice to tackle chronic kidney disease (CKD) for thousands of years. Nowadays, many studies indicate that gut bacteria are closely related to the progression of CKD and CKD‐related complications. In this study, a UP...

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Bibliographic Details
Published in:Biomedical chromatography 2017-02, Vol.31 (2), p.np-n/a
Main Authors: Du, Le‐yue, Tao, Jin‐hua, Jiang, Shu, Qian, Da‐wei, Guo, Jian‐ming, Duan, Jin‐ao
Format: Article
Language:English
Subjects:
Abelmoschus
Abelmoschus manihot
Animals
Bacteria
Chromatography, High Pressure Liquid - methods
chronic kidney disease
Drugs, Chinese Herbal - chemistry
Drugs, Chinese Herbal - metabolism
Flavonoids - analysis
Flavonoids - metabolism
Flos Abelmoschus manihot
gut microbiome
Intestines - metabolism
Intestines - microbiology
Male
Malvaceae - chemistry
Manihot
Mass Spectrometry - methods
metabolism
Metabolome
methylation
Quercetin - analysis
Quercetin - metabolism
Rats
Rats, Sprague-Dawley
Renal Insufficiency, Chronic - metabolism
Renal Insufficiency, Chronic - microbiology
UPLC‐Q‐TOF/MS
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Summary:Flos Abelmoschus manihot is a traditional herbal medicine widely used in clinical practice to tackle chronic kidney disease (CKD) for thousands of years. Nowadays, many studies indicate that gut bacteria are closely related to the progression of CKD and CKD‐related complications. In this study, a UPLC‐Q‐TOF/MS method coupled with the MetaboLynx™ software was established and successfully applied to investigate the metabolites and metabolic profile of Flos A. manihot extract by intestinal bacteria from normal and CKD rats. Eight parent components and eight metabolites were characterized by their protonated ions. Among these compounds, 15 were detected in the two group samples while M16 was only determined in the CKD model samples. Compared with the quercetin‐type glycosides, fewer myricetin‐type and gossypetin‐type metabolites were obtained in the two group samples. These metabolites suggested that deglycosylation and methylation are the major metabolic pathways of Flos A. manihot extract. Few differences of metabolite classes were observed in the two group samples. However, the concentrations of aglycones such as quercetin, myricetin and gossypetin in the normal samples were notably higher than those in the CKD model samples. The results are important in unravelling the pharmacological effects of A. manihot and clarifying its mechanism of action in vivo.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.3795