Histopathological assessment of chronic Corynebacterium pseudotuberculosis infection in the reproductive tract and iliac lymph node of Katjang does

• Published in: Comparative clinical pathology 2017, Vol.26 (1), p.147-154
• Main Authors: Latif, Nur Amirah Abdul, Abba, Yusuf, Jesse, Faez Firdaus Abdullah, Chung, Eric Lim Teik, Zamri-Saad, Mohd, Saharee, Abdul Aziz, Zakaria, Zunita, Haron, Abdul Wahid, Mohd-Lila, Mohd Azmi
• Format: Article
• Language: English
• Subjects: Corynebacterium pseudotuberculosis; Hematology; Medicine; Medicine & Public Health; Oncology; Original Article; Pathology; Ruminantia
• ISSN: 1618-5641; 1618-565X
• DOI: 10.1007/s00580-016-2357-3

Corynebacterium pseudotuberculosis is the etiological agent for caseous lymphadenitis (CLA), which is a chronic zoonotic disease that mostly infects sheep and goats worldwide. CLA causes significant financial losses in endemic countries due to decreased productivity and impaired reproduction in goats, sheep, and other small ruminants. In this study, 18 non-pregnant healthy Katjang does aged 2 years old were randomly divided into two groups of nine goats each. The first group were all experimentally inoculated with 1 ml of 10 7  cfu of live C. pseudotuberculosis through intradermal route while the second group was given 1 ml PBS (pH 7) intradermally. Three animals from each group were withdrawn and culled after 30, 60 and 90 days post infection and the reproductive organs (vagina, cervix, uterus, uterine horns, and ovaries) and iliac lymph nodes were collected in 10 % buffered formalin, processed, sectioned, and stained with H&E. Histopathological findings showed evidence of inflammation, degeneration, necrosis, and vascular changes in all the tissues examined. Inflammatory cell infiltration made up of neutrophils and macrophages were observed in all tissues. However, while neutrophil response was higher ( p