Biologic variability of N-terminal pro-brain natriuretic peptide in adult healthy cats

• Published in: Journal of feline medicine and surgery 2017-02, Vol.19 (2), p.216-223
• Main Authors: Harris, Autumn N, Estrada, Amara H, Gallagher, Alexander E, Winter, Brandy, Lamb, Kenneth E, Bohannon, Mary, Hanscom, Jancy, Mainville, Celine A
• Format: Article
• Language: English
• Subjects: Animals; Biological Products; Biomarkers - blood; Cats - blood; Echocardiography - veterinary; Electrocardiography; Female; Male; Natriuretic Peptide, Brain - blood; Peptide Fragments - blood; Reference Values; Sensitivity and Specificity
• ISSN: 1098-612X; 1532-2750; 1532-2750
• DOI: 10.1177/1098612X15623825

Objectives The biologic variability of N-terminal pro-brain natriuretic peptide (NT-proBNP) and its impact on diagnostic utility is unknown in healthy cats and those with cardiac disease. The purpose of this study was to determine the biologic variation of NT-proBNP within-day and week-to-week in healthy adult cats. Methods Adult cats were prospectively evaluated by complete blood count (CBC), biochemistry, total thyroxine, echocardiography, electrocardiography and blood pressure, to exclude underlying systemic or cardiac disease. Adult healthy cats were enrolled and blood samples were obtained at 11 time points over a 6 week period (0, 2 h, 4 h, 6 h, 8 h, 10 h and at weeks 2, 3, 4, 5 and 6). The intra-individual (coefficient of variation [CVI]) biologic variation along with index of individuality and reference change values (RCVs) were calculated. Univariate models were analyzed and included comparison of the six different time points for both daily and weekly samples. This was followed by a Tukey’s post-hoc adjustment, with a P value of