Prenylated isoflavones from Cudrania tricuspidata inhibit NO production in RAW 264.7 macrophages and suppress HL-60 cells proliferation

• Published in: Journal of asian natural products research 2017-05, Vol.19 (5), p.510-518
• Main Authors: Tuan Anh, Hoang Le, Tuan, Do Thanh, Trang, Do Thi, Tai, Bui Huu, Nhiem, Nguyen Xuan, Yen, Pham Hai, Kiem, Phan Van, Minh, Chau Van, Duc, Tran Minh, Kang, Hee Kyoung, Kim, Youn Chul, Kim, Young Ho
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal - chemistry; Anti-Inflammatory Agents, Non-Steroidal - isolation & purification; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; anti-leukemia; cudraisoflavone L; Cudrania tricuspidata; Cytoprotection - drug effects; HL-60 Cells; Humans; Isoflavones - chemistry; Isoflavones - isolation & purification; Isoflavones - pharmacology; Macrophages - drug effects; Mice; Molecular Structure; Moraceae - chemistry; Nitric Oxide - biosynthesis; NO inhibitor; Plant Roots - chemistry; Prenylated isoflavone; Prenylation
• ISSN: 1028-6020; 1477-2213
• DOI: 10.1080/10286020.2016.1232253

Inhibitory effects of NO production in RAW 264.7 macrophages guided the isolation of nine prenylated isoflavones, including a new cudraisoflavone L (1) and eight known metabolites furowanin B (2), erysubin A (3), wighteone (4), lupalbigenin (5), laburnetin (6), isolupalbigenin (7), 6,8-diprenylorobol (8), millewanin H (9) from the leaves of Cudrania tricuspidata. At the concentration of 10 μM, compounds 1, 2, and 4 significantly inhibited NO production with the inhibitory values of 72.5 ± 2.4, 66.9 ± 1.8, and 55.4 ± 2.7%, respectively. In addition, all of isolated compounds 1-9 showed promising cytotoxic effects toward HL-60 cells (IC 50 4.3 ± 0.7 to 18.0 ± 1.7 μM).