Suitability of the Fluorescence Monitoring System (FMS, Hansatech) for measurement of photosynthetic characteristics in algae

A pulse-modulated fluorescence measuring system, originally developed for higher plant studies, the Fluorescence Monitoring System (FMS; Hansatech), was tested to study photosynthesis in algae. The main technical features of the FMS are described, and applications of the saturating pulse fluorescenc...

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Bibliographic Details
Published in:Aquatic botany 2002-11, Vol.74 (3), p.219-231
Main Authors: Mouget, Jean-Luc, Tremblin, Gérard
Format: Article
Language:English
Subjects:
Chlorophyll fluorescence
Fucus serratus
Macroalgae
Microalgae
Modulation fluorometer
Palmaria palmata
Quenching analysis
Ulva
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Summary:A pulse-modulated fluorescence measuring system, originally developed for higher plant studies, the Fluorescence Monitoring System (FMS; Hansatech), was tested to study photosynthesis in algae. The main technical features of the FMS are described, and applications of the saturating pulse fluorescence method are presented on micro- and macroalgae. Some limitations of the apparatus for measuring fluorescence characteristics of microalgae are discussed. Chlorophyll a fluorescence quenching analyses were performed on macroalgae from different taxonomic groups ( Ulva sp., Palmaria palmata, Fucus serratus), and the results of the fluorescence measurements are presented and discussed, in relation to the macroalgae characteristics. The FMS and the saturating pulse method may be successfully used with green and red algae, in contrast to brown algae, with which F m′ values higher than F m were observed at low actinic light (
ISSN:0304-3770
1879-1522
DOI:10.1016/S0304-3770(02)00104-3