Cloth-based hybridization array system for detection of transgenic soy and corn by multiplex polymerase chain reaction

A cloth-based hybridization array system (CHAS) was developed for detection of amplicons generated in a multiplex PCR targeting transgenic 35S and NOS sequences, as well as corn invertase and soy lectin genes. The CHAS provided confirmation of each amplicon on the basis of hybridization with specifi...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 2002-09, Vol.24 (17), p.1407-1411
Main Authors: RINK, Burton W, PHILLIPPE, Lucille M, VARY, Neil
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Corn
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Hybridization
Methods. Procedures. Technologies
Microbiology
Transgenic animals and transgenic plants
Transgenic plants
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A cloth-based hybridization array system (CHAS) was developed for detection of amplicons generated in a multiplex PCR targeting transgenic 35S and NOS sequences, as well as corn invertase and soy lectin genes. The CHAS provided confirmation of each amplicon on the basis of hybridization with specific capture probes, and enabled ease of discrimination of the multiplex PCR products by visualization of the amplicons on the array.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1019894330731