Phylogeographic structure of Canthon cyanellus (Coleoptera: Scarabaeidae), a Neotropical dung beetle in the Mexican Transition Zone: Insights on its origin and the impacts of Pleistocene climatic fluctuations on population dynamics

• Published in: Molecular phylogenetics and evolution 2017-04, Vol.109, p.180-190
• Main Authors: Nolasco-Soto, Janet, González-Astorga, Jorge, Espinosa de los Monteros, Alejandro, Galante-Patiño, Eduardo, Favila, Mario E.
• Format: Article
• Language: English
• Subjects: Animals; Cell Nucleus - genetics; Coleoptera - classification; Coleoptera - genetics; DNA, Mitochondrial - genetics; Genetic Loci; Genetic Variation; Haplotypes; Historical demography; Mexico; Neotropics; Phylogeny; Phylogeography; Pleistocene glaciations; Population divergence; Population Dynamics; Time Factors; Tropical Climate
• ISSN: 1055-7903; 1095-9513
• DOI: 10.1016/j.ympev.2017.01.004

[Display omitted] •Phylogeographic analyses of Canthon cyanellus in Mexico reveals lineages historical differentiation.•The divergence of this Neotropical group occurred during the Pleistocene.•Geological events and glacial cycles influenced the geographic structure of the lineages.•The demographic history suggests post-glacial expansions.•Not all the current valid subspecies are supported by the molecular analysis. Canthon cyanellus is a roller dung beetle with a wide distribution range in the tropical forests of the New World. In Mexico, it inhabits the Pacific and the Gulf coasts, the Yucatan Peninsula and the south mainly in the State of Chiapas. This species shows a wide geographical variation in cuticle color, which has been used as defining trait for subspecies. In this study we analyzed the phylogeographic and demographic history of the Mexican populations of C. cyanellus using DNA sequences of the nuclear ITS2, and the mitochondrial COI and 16S genes. We found that not all the current valid subspecies are supported by the molecular analysis. The populations are genetically and geographically structured in five lineages. The diversification events that gave origin to the main lineages within this species complex occurred during the Pleistocine in a time range of 1.63–0.91Myr. The demographic history of these lineages suggests post-glacial expansions toward the middle and the end of the Pleistocene. The combined data of mitochondrial and nuclear DNA suggest that the phylogeographic structure and demographic history of the C. cyanellus populations are the result of: the geological and volcanic activity that occurred from the end of the Pliocene to the Pleistocene; and the contraction and expansion of tropical forests due to the glacial and inter-glacial cycles during the Pleistocene. Landscape changes derived from historical events have affected the demographic history of the populations of this species. The results presented here point to the need to review the taxonomic status and delimitation of the lineages encompassed in the Canthon cyanellus complex.