Extremely high intracellular concentration of glucose-6-phosphate and NAD(H) in Deinococcus radiodurans

Deinococcus radiodurans is highly resistant to ionizing radiation and UV radiation, and oxidative stress caused by such radiations. NADP(H) seems to be important for this resistance (Slade and Radman, Microbiol Mol Biol Rev 75:133–191; Slade, Radman, Microbiol Mol Biol Rev 75:133–191, 2011), but the...

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Bibliographic Details
Published in:Extremophiles : life under extreme conditions 2017-03, Vol.21 (2), p.399-407
Main Authors: Yamashiro, Takumi, Murata, Kousaku, Kawai, Shigeyuki
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - metabolism
Biochemistry
Biomedical and Life Sciences
Biotechnology
Deinococcus - metabolism
Enzymes
Glucose-6-Phosphate - metabolism
Glucosephosphate Dehydrogenase - metabolism
Ionizing radiation
Life Sciences
Microbial Ecology
Microbiology
NAD - metabolism
Original Paper
Oxidative stress
Radiation
Space life sciences
Ultraviolet radiation
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Summary:Deinococcus radiodurans is highly resistant to ionizing radiation and UV radiation, and oxidative stress caused by such radiations. NADP(H) seems to be important for this resistance (Slade and Radman, Microbiol Mol Biol Rev 75:133–191; Slade, Radman, Microbiol Mol Biol Rev 75:133–191, 2011), but the mechanism underlying the generation of NADP(H) or NAD(H) in D. radiodurans has not fully been addressed. Intracellular concentrations of NAD + , NADH, NADP + , and NADPH in D. radiodurans are also not determined yet. We found that cell extracts of D. radiodurans catalyzed reduction of NAD(P) + in vitro, indicating that D. radiodurans cells contain both enzymes and a high concentration of substrates for this activity. The enzyme and the substrate were attributed to glucose-6-phosphate dehydrogenase and glucose-6-phosphate of which intracellular concentration was extremely high. Unexpectedly, the intracellular concentration of NAD(H) was also much greater than that of NADP(H), suggesting some significant roles of NADH. These unusual features of this bacterium would shed light on a new aspect of physiology of this bacterium.
ISSN:1431-0651
1433-4909
DOI:10.1007/s00792-016-0913-z