Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model

To explore the possible efficacy of electromagnetic fields (EMF) for skin tissue engineering, effects of EMF exposure on epidermal stem cells (ESC) seeded in collagen sponge scaffolds for wound healing in a murine model were investigated. The wound models of a full‐thickness defect established with...

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Published in:Bioelectromagnetics 2017-04, Vol.38 (3), p.204-212
Main Authors: Bai, Wen‐Fang, Xu, Wei‐Cheng, Zhu, Hong‐Xiang, Huang, Hong, Wu, Bo, Zhang, Ming‐Sheng
Format: Article
Language:English
Subjects:
Animals
Biomimetic Materials
Cell Culture Techniques
Collagen Type I
Electromagnetic Fields
epidermal stem cells
Epidermis - cytology
Humans
Male
Mice, Nude
scaffold
Stem Cell Transplantation - methods
Stem Cells - cytology
Stem Cells - metabolism
Tissue Engineering - methods
Tissue Scaffolds
transplantation
wound healing
Wound Healing - physiology
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cited_by cdi_FETCH-LOGICAL-c3259-4ed725fa75de013728d0f3919f16e426a5177424950ef92735cd0bb409420c3c3
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container_end_page 212
container_issue 3
container_start_page 204
container_title Bioelectromagnetics
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creator Bai, Wen‐Fang
Xu, Wei‐Cheng
Zhu, Hong‐Xiang
Huang, Hong
Wu, Bo
Zhang, Ming‐Sheng
description To explore the possible efficacy of electromagnetic fields (EMF) for skin tissue engineering, effects of EMF exposure on epidermal stem cells (ESC) seeded in collagen sponge scaffolds for wound healing in a murine model were investigated. The wound models of a full‐thickness defect established with 36 7 ∼ 8‐week‐old nude mice were randomly divided into three groups: a control group, an ESC‐only group, and an ESC with EMF exposure group (frequency of 50 Hz, magnetic induction of 5 mT, 60 min per day for 20 days). ESC were separated from human foreskin and cultured in vitro, and then transplanted with collagen sponge scaffolds as a delivery vehicle to wounds of the ESC‐only group, and ESC with EMF exposure group was exposed to EMF after ESC transplantation. Effects of EMF on morphological changes and expression of β1 integrin in regenerated skins were observed. Wound healing rates and healing times were collected to evaluate the efficacy of repairment. Results showed that human ESC were successfully transplanted to nude mice, which facilitated the formation of intact skin on nude mice. In contrast to other groups, the wound healing of ESC with EMF exposure group was the fastest (P 
doi_str_mv 10.1002/bem.22029
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The wound models of a full‐thickness defect established with 36 7 ∼ 8‐week‐old nude mice were randomly divided into three groups: a control group, an ESC‐only group, and an ESC with EMF exposure group (frequency of 50 Hz, magnetic induction of 5 mT, 60 min per day for 20 days). ESC were separated from human foreskin and cultured in vitro, and then transplanted with collagen sponge scaffolds as a delivery vehicle to wounds of the ESC‐only group, and ESC with EMF exposure group was exposed to EMF after ESC transplantation. Effects of EMF on morphological changes and expression of β1 integrin in regenerated skins were observed. Wound healing rates and healing times were collected to evaluate the efficacy of repairment. Results showed that human ESC were successfully transplanted to nude mice, which facilitated the formation of intact skin on nude mice. In contrast to other groups, the wound healing of ESC with EMF exposure group was the fastest (P &lt; 0.05), the structure of regenerated skins was more mature, and it contained more continuity in the number of viable cell layers and rich hair follicles’ structure. These results suggest that the use of 50 Hz EMF as a non‐invasive treatment can accelerate wound healing of ESC transplantation, and restore structural integrity of regenerated skin. 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In contrast to other groups, the wound healing of ESC with EMF exposure group was the fastest (P &lt; 0.05), the structure of regenerated skins was more mature, and it contained more continuity in the number of viable cell layers and rich hair follicles’ structure. These results suggest that the use of 50 Hz EMF as a non‐invasive treatment can accelerate wound healing of ESC transplantation, and restore structural integrity of regenerated skin. Bioelectromagnetics. 38:204–212,2017. © 2017 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pmid>28106913</pmid><doi>10.1002/bem.22029</doi><tpages>9</tpages></addata></record>
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source Wiley-Blackwell Read & Publish Collection
subjects Animals
Biomimetic Materials
Cell Culture Techniques
Collagen Type I
Electromagnetic Fields
epidermal stem cells
Epidermis - cytology
Humans
Male
Mice, Nude
scaffold
Stem Cell Transplantation - methods
Stem Cells - cytology
Stem Cells - metabolism
Tissue Engineering - methods
Tissue Scaffolds
transplantation
wound healing
Wound Healing - physiology
title Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model
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