Development of a high-throughput opsonophagocytic assay for the determination of functional antibody activity against Streptococcus pyogenes using bioluminescence

The lack of standardised protocols for the assessment of functional antibodies has hindered Streptococcus pyogenes research and the development of vaccines. A robust, high throughput opsonophagocytic bactericidal assay to determine protective antibodies in human and rabbit serum has been developed t...

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Bibliographic Details
Published in:Journal of microbiological methods 2017-03, Vol.134, p.58-61
Main Authors: Lorenz, Natalie, Loh, Jacelyn M.S., Moreland, Nicole J., Proft, Thomas
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - blood
Antibodies, Bacterial - immunology
Bactericidal assay
Bioluminescence
Correlates of protection
Enzyme-Linked Immunosorbent Assay
Group A Streptococcus (GAS)
High-Throughput Screening Assays
Humans
Immunologic Tests - methods
Lancefield assay
Luminescent Measurements
Phagocytosis
Protective antibodies
Rabbits
Streptococcal Infections - diagnosis
Streptococcal Infections - microbiology
Streptococcus pyogenes - immunology
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Summary:The lack of standardised protocols for the assessment of functional antibodies has hindered Streptococcus pyogenes research and the development of vaccines. A robust, high throughput opsonophagocytic bactericidal assay to determine protective antibodies in human and rabbit serum has been developed that utilises bioluminescence as a rapid read out.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2017.01.010