Micropatterning of nanocomposite polymer scaffolds using sacrificial phosphate glass fibers for tendon tissue engineering applications

Abstract This study presents a simple and reproducible method of micropatterning the novel nanocomposite polymer (POSS-PCU) using a sacrificial phosphate glass fiber template for tendon tissue engineering applications. The diameters of the patterned scaffolds produced were dependent on the diameter...

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Bibliographic Details
Published in:Nanomedicine 2017-04, Vol.13 (3), p.1267-1277
Main Authors: Alshomer, Feras, MBBS, Chaves, Camilo, MBBS, MSc, Serra, Tiziano, PhD, Ahmed, Ifty, PhD, Kalaskar, Deepak M., PhD
Format: Article
Language:English
Subjects:
Cell Proliferation
Cells, Cultured
Collagen - analysis
Collagen - metabolism
Glass - chemistry
Humans
Internal Medicine
Male
Micropattern scaffolds
Middle Aged
Nanocomposite polymer
Nanocomposites - chemistry
Phosphate glass fibers
Phosphates - chemistry
Tendon graft
Tendons - cytology
Tendons - metabolism
Tissue Engineering - methods
Tissue Scaffolds - chemistry
Wettability
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Summary:Abstract This study presents a simple and reproducible method of micropatterning the novel nanocomposite polymer (POSS-PCU) using a sacrificial phosphate glass fiber template for tendon tissue engineering applications. The diameters of the patterned scaffolds produced were dependent on the diameter of the glass fibers (15 μm) used. Scaffolds were tested for their physical properties and reproducibility using various microscopy techniques. For the first time, we show that POSS-PCU supports growth of human tenocytes cells. Furthermore, we show that cellular alignment, their biological function and expression of various tendon related proteins such as scleraxis, collagen I and III, tenascin-C are significantly elevated on the micropatterned polymer surfaces compared to flat samples. This study demonstrated a simple, reproducible method of micropatterning POSS-PCU nanocomposite polymer for novel tendon repair applications, which when provided with physical cues could help mimic the microenvironment of tenocytes cells.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2017.01.006