Molecular characterization of CD44+/CD24−/Ck+/CD45− cells in benign and malignant breast lesions

• Published in: Virchows Archiv : an international journal of pathology 2017-03, Vol.470 (3), p.311-322
• Main Authors: Da Cruz Paula, Arnaud, Leitão, Catarina, Marques, Oriana, Rosa, Ana Margarida, Santos, Ana Helena, Rêma, Alexandra, de Fátima Faria, Maria, Rocha, Ana, Costa, José Luís, Lima, Margarida, Lopes, Carlos
• Format: Article
• Language: English
• Subjects: Biomarkers, Tumor - analysis; Breast Diseases - genetics; Breast Diseases - mortality; Breast Diseases - pathology; Breast Neoplasms - genetics; Breast Neoplasms - mortality; Breast Neoplasms - pathology; Cadherins - analysis; Cadherins - biosynthesis; Carcinoma, Ductal, Breast - genetics; Carcinoma, Ductal, Breast - mortality; Carcinoma, Ductal, Breast - pathology; Carcinoma, Intraductal, Noninfiltrating - genetics; Carcinoma, Intraductal, Noninfiltrating - mortality; Carcinoma, Intraductal, Noninfiltrating - pathology; DNA Mutational Analysis; Female; Flow Cytometry; High-Throughput Nucleotide Sequencing; Humans; Hyaluronan Receptors - analysis; Hyaluronan Receptors - biosynthesis; Immunohistochemistry; Kaplan-Meier Estimate; Leukocyte Common Antigens - analysis; Leukocyte Common Antigens - biosynthesis; Medicine; Medicine & Public Health; Neoplastic Stem Cells - pathology; Original Article; Pathology; Phenotype
• ISSN: 0945-6317; 1432-2307
• DOI: 10.1007/s00428-017-2068-4

Breast cancer epithelial cells with the CD44 + /CD24 −/low phenotype possess tumor-initiating cells and epithelial-mesenchymal transition (EMT) capacity. Massive parallel sequencing can be an interesting approach to deepen the molecular characterization of these cells. We characterized CD44 + /CD24 − /cytokeratin(Ck) + /CD45 − cells isolated through flow cytometry from 43 biopsy and 6 mastectomy samples harboring different benign and malignant breast lesions. The Ion Torrent Ampliseq Cancer Hotspot panel v2 (CHPv2) was used for the identification of somatic mutations in the DNA extracted from isolated CD44 + /CD24 − /Ck + /CD45 − cells. E-Cadherin and vimentin immunohistochemistry was performed on sections from the corresponding formalin-fixed, paraffin-embedded (FFPE) blocks. The percentage of CD44 + /CD24 − /Ck + /CD45 − cells increased significantly from non-malignant to malignant lesions and in association with a significant increase in the expression of vimentin. Non-malignant lesions harbored only a single-nucleotide polymorphism (SNP). Mutations in the tumor suppressor p53 ( TP53 ), NOTCH homolog 1 ( NOTCH1 ), phosphatase and tensin homolog ( PTEN ), and v-akt murine thymoma viral oncogene homolog 1 ( AKT1 ) genes were found in isolated CD44 + /CD24 − /Ck + /CD45 − cells from ductal carcinomas in situ (DCIS). Additional mutations in the colony-stimulating factor 1 receptor ( CSF1R ), ret proto-oncogene ( RET ), and TP53 genes were also identified in invasive ductal carcinomas (IDCs). The use of massive parallel sequencing technology for this type of application revealed to be extremely effective even when using small amounts of DNA extracted from a low number of cells. Additional studies are now required using larger cohorts to design an appropriate mutational profile for this phenotype.