In vivo near-infrared imaging and phototherapy of tumors using a cathepsin B-activated fluorescent probe

Abstract The development of multifunctional reagents for simultaneous specific near-infrared (NIR) imaging and phototherapy of tumors is of great significance. This work describes the design of a cathepsin B-activated fluorescent probe ( CyA-P-CyB ) and its applications as an NIR imaging probe for t...

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Bibliographic Details
Published in:Biomaterials 2017-04, Vol.122, p.130-140
Main Authors: Chen, Xiaoqiang, Lee, Dayoung, Yu, Sungsook, Kim, Gyoungmi, Lee, Songyi, Cho, Yejin, Jeong, Haengdueng, Nam, Ki Taek, Yoon, Juyoung
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Cathepsin B
Cell Line, Tumor
Dentistry
Enzyme-activated fluorescence
Fluorescent Dyes - chemical synthesis
Humans
Infrared Rays
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Microscopy, Fluorescence - methods
Multiple functional probe
Neoplasms, Experimental - drug therapy
Neoplasms, Experimental - metabolism
Neoplasms, Experimental - pathology
NIR bioimaging
Oligopeptides - chemistry
Oligopeptides - pharmacokinetics
Photochemotherapy - methods
Photosensitizing Agents - administration & dosage
Specific phototherapy
Theranostic Nanomedicine - methods
Treatment Outcome
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Summary:Abstract The development of multifunctional reagents for simultaneous specific near-infrared (NIR) imaging and phototherapy of tumors is of great significance. This work describes the design of a cathepsin B-activated fluorescent probe ( CyA-P-CyB ) and its applications as an NIR imaging probe for tumor cells and as a phototherapy reagent for tumors. In vitro experiments demonstrated that CyA-P-CyB was activated via the cleavage of a peptide linker by cathepsin B in tumor cells to produce fluorescence in the NIR region based on a FRET mechanism. MTT assays showed that the phototoxicity of CyA-P-CyB toward cells depended on the activity of cathepsin B, and the probe exhibited specific phototoxicity toward tumor cells. CyA-P-CyB was also successfully applied to the in vivo imaging and phototherapy of tumors. Histological analysis indicated that CyA-P-CyB had no cytotoxic effects on seven mouse tissues (lung, liver, heart, kidney, pancreas, spleen and brain) after the CyA-P-CyB treatment and laser irradiation.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2017.01.020