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Relationship between PDK1 and contraction in carotid arteries in Goto-Kakizaki rat, a spontaneous type 2 diabetic animal model
We studied the relationship between 3-phosphoinositide-dependent protein kinase 1 (PDK1) and contractions induced by serotonin, phenylephrine, and thromboxane A 2 mimetic (U46619) in the presence of nitric oxide synthase inhibitor in the carotid arteries of Goto-Kakizaki (GK) rats, a spontaneous typ...
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| Published in: | Canadian journal of physiology and pharmacology 2017-04, Vol.95 (4), p.459-462 |
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| cites | cdi_FETCH-LOGICAL-c631t-cf8e6a8ac4ea9b97870ac715b9f832dff0ddb9e7fdb3c1d66d3a8590ff4ec203 |
| container_end_page | 462 |
| container_issue | 4 |
| container_start_page | 459 |
| container_title | Canadian journal of physiology and pharmacology |
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| creator | Watanabe, Shun Matsumoto, Takayuki Taguchi, Kumiko Kobayashi, Tsuneo |
| description | We studied the relationship between 3-phosphoinositide-dependent protein kinase 1 (PDK1) and contractions induced by serotonin, phenylephrine, and thromboxane A
2
mimetic (U46619) in the presence of nitric oxide synthase inhibitor in the carotid arteries of Goto-Kakizaki (GK) rats, a spontaneous type 2 diabetic model, in the chronic stage of disease. Serotonin-induced contraction was greater in the GK rats than in the control Wistar rats. A specific PDK1 inhibitor (GSK2334470) decreased the serotonin-induced contraction in the GK rats but not in the Wistar rats, and the difference in such contraction was abolished with this treatment. In GK rats, phenylephrine-induced contraction exhibited a leftward shift and U46619-induced contraction was greater still. Phenylephrine- and U46619-induced contractions were reduced by GSK2334470 in both groups. These results suggest, for the first time, that the contribution of PDK1 is different among 3 vasoconstrictors and that PDK1 contributed to increased serotonin-induced contraction in the carotid arteries of GK rats. |
| doi_str_mv | 10.1139/cjpp-2016-0372 |
| format | article |
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2
mimetic (U46619) in the presence of nitric oxide synthase inhibitor in the carotid arteries of Goto-Kakizaki (GK) rats, a spontaneous type 2 diabetic model, in the chronic stage of disease. Serotonin-induced contraction was greater in the GK rats than in the control Wistar rats. A specific PDK1 inhibitor (GSK2334470) decreased the serotonin-induced contraction in the GK rats but not in the Wistar rats, and the difference in such contraction was abolished with this treatment. In GK rats, phenylephrine-induced contraction exhibited a leftward shift and U46619-induced contraction was greater still. Phenylephrine- and U46619-induced contractions were reduced by GSK2334470 in both groups. These results suggest, for the first time, that the contribution of PDK1 is different among 3 vasoconstrictors and that PDK1 contributed to increased serotonin-induced contraction in the carotid arteries of GK rats.</description><identifier>ISSN: 0008-4212</identifier><identifier>EISSN: 1205-7541</identifier><identifier>DOI: 10.1139/cjpp-2016-0372</identifier><identifier>PMID: 28118732</identifier><identifier>CODEN: CJPPA3</identifier><language>eng</language><publisher>Canada: NRC Research Press</publisher><subject>15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid - pharmacology ; 3-Phosphoinositide-Dependent Protein Kinases - antagonists & inhibitors ; 3-Phosphoinositide-Dependent Protein Kinases - metabolism ; Animals ; artère carotide ; Carotid arteries ; Carotid Arteries - drug effects ; Carotid Arteries - physiology ; carotid artery ; Chronic Disease ; contraction ; Diabetes ; Diabetes Mellitus, Type 2 - complications ; Diabetic Angiopathies - drug therapy ; Diabetic Angiopathies - etiology ; Diabetic Angiopathies - metabolism ; diabète de type 2 ; Disease Models, Animal ; GK rat ; Health aspects ; Indazoles - pharmacology ; Kinases ; Male ; Nitric Oxide - metabolism ; Nitric Oxide Synthase - antagonists & inhibitors ; PDK1 ; Phenylephrine - pharmacology ; Protein kinases ; Pyrimidines - pharmacology ; rat GK ; Rats ; Rats, Wistar ; Rodents ; Serotonin ; Serotonin - pharmacology ; Signal Transduction ; Thromboxane A2 - analogs & derivatives ; Type 2 diabetes ; Vasoconstriction - drug effects ; Vasoconstrictor Agents - pharmacology</subject><ispartof>Canadian journal of physiology and pharmacology, 2017-04, Vol.95 (4), p.459-462</ispartof><rights>COPYRIGHT 2017 NRC Research Press</rights><rights>Copyright Canadian Science Publishing NRC Research Press Apr 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c631t-cf8e6a8ac4ea9b97870ac715b9f832dff0ddb9e7fdb3c1d66d3a8590ff4ec203</citedby><cites>FETCH-LOGICAL-c631t-cf8e6a8ac4ea9b97870ac715b9f832dff0ddb9e7fdb3c1d66d3a8590ff4ec203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28118732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Watanabe, Shun</creatorcontrib><creatorcontrib>Matsumoto, Takayuki</creatorcontrib><creatorcontrib>Taguchi, Kumiko</creatorcontrib><creatorcontrib>Kobayashi, Tsuneo</creatorcontrib><title>Relationship between PDK1 and contraction in carotid arteries in Goto-Kakizaki rat, a spontaneous type 2 diabetic animal model</title><title>Canadian journal of physiology and pharmacology</title><addtitle>Can J Physiol Pharmacol</addtitle><description>We studied the relationship between 3-phosphoinositide-dependent protein kinase 1 (PDK1) and contractions induced by serotonin, phenylephrine, and thromboxane A
2
mimetic (U46619) in the presence of nitric oxide synthase inhibitor in the carotid arteries of Goto-Kakizaki (GK) rats, a spontaneous type 2 diabetic model, in the chronic stage of disease. Serotonin-induced contraction was greater in the GK rats than in the control Wistar rats. A specific PDK1 inhibitor (GSK2334470) decreased the serotonin-induced contraction in the GK rats but not in the Wistar rats, and the difference in such contraction was abolished with this treatment. In GK rats, phenylephrine-induced contraction exhibited a leftward shift and U46619-induced contraction was greater still. Phenylephrine- and U46619-induced contractions were reduced by GSK2334470 in both groups. These results suggest, for the first time, that the contribution of PDK1 is different among 3 vasoconstrictors and that PDK1 contributed to increased serotonin-induced contraction in the carotid arteries of GK rats.</description><subject>15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid - pharmacology</subject><subject>3-Phosphoinositide-Dependent Protein Kinases - antagonists & inhibitors</subject><subject>3-Phosphoinositide-Dependent Protein Kinases - metabolism</subject><subject>Animals</subject><subject>artère carotide</subject><subject>Carotid arteries</subject><subject>Carotid Arteries - drug effects</subject><subject>Carotid Arteries - physiology</subject><subject>carotid artery</subject><subject>Chronic Disease</subject><subject>contraction</subject><subject>Diabetes</subject><subject>Diabetes Mellitus, Type 2 - complications</subject><subject>Diabetic Angiopathies - drug therapy</subject><subject>Diabetic Angiopathies - etiology</subject><subject>Diabetic Angiopathies - metabolism</subject><subject>diabète de type 2</subject><subject>Disease Models, Animal</subject><subject>GK rat</subject><subject>Health aspects</subject><subject>Indazoles - pharmacology</subject><subject>Kinases</subject><subject>Male</subject><subject>Nitric Oxide - metabolism</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>PDK1</subject><subject>Phenylephrine - pharmacology</subject><subject>Protein kinases</subject><subject>Pyrimidines - pharmacology</subject><subject>rat GK</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Serotonin</subject><subject>Serotonin - pharmacology</subject><subject>Signal Transduction</subject><subject>Thromboxane A2 - analogs & derivatives</subject><subject>Type 2 diabetes</subject><subject>Vasoconstriction - drug effects</subject><subject>Vasoconstrictor Agents - pharmacology</subject><issn>0008-4212</issn><issn>1205-7541</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNqVkttrFDEUxgdR7Fp99VGCvig4NZe5ZB5L1VpaVGrfQyY52WadTaZJBq0P_u1m2PWysiASQsjhdz4-zvmK4jHBR4Sw7pVajWNJMWlKzFp6p1gQiuuyrStyt1hgjHlZUUIPigcxrvK34YzfLw4oJ4S3jC6K75cwyGS9i9d2RD2kLwAOfXx9TpB0GinvUpBqBpB1SMngk9VIhgTBQpxrpz758lx-tt_yRUGml0iiOOZG6cBPEaXbERBF2sosb1XWtWs5oLXXMDws7hk5RHi0fQ-Lq7dvrk7elRcfTs9Oji9K1TCSSmU4NJJLVYHs-q7lLZaqJXXfGc6oNgZr3XfQGt0zRXTTaCZ53WFjKlAUs8Pi-UZ2DP5mgpjE2kYFw7CxKAhvCK9ZRXhGn_2FrvwUXDaXKd40LccV-00t5QDCOuPnMc2i4rjqcMebltaZKvdQS3AQ5OAdGJvLO_zTPbwa7Y34EzraA-WjYW3VXtUXOw3zTuFrWsopRnH26fI_2Pe77NaICj7GAEaMIW823AqCxRxOMYdTzOEUczhzw5PtaKd-DfoX_jONGSAbwAUVIIIM6vpfoj8AZb_tBg</recordid><startdate>20170401</startdate><enddate>20170401</enddate><creator>Watanabe, Shun</creator><creator>Matsumoto, Takayuki</creator><creator>Taguchi, Kumiko</creator><creator>Kobayashi, Tsuneo</creator><general>NRC Research Press</general><general>Canadian Science Publishing NRC Research Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISN</scope><scope>ISR</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20170401</creationdate><title>Relationship between PDK1 and contraction in carotid arteries in Goto-Kakizaki rat, a spontaneous type 2 diabetic animal model</title><author>Watanabe, Shun ; Matsumoto, Takayuki ; Taguchi, Kumiko ; Kobayashi, Tsuneo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c631t-cf8e6a8ac4ea9b97870ac715b9f832dff0ddb9e7fdb3c1d66d3a8590ff4ec203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid - pharmacology</topic><topic>3-Phosphoinositide-Dependent Protein Kinases - antagonists & inhibitors</topic><topic>3-Phosphoinositide-Dependent Protein Kinases - metabolism</topic><topic>Animals</topic><topic>artère carotide</topic><topic>Carotid arteries</topic><topic>Carotid Arteries - drug effects</topic><topic>Carotid Arteries - physiology</topic><topic>carotid artery</topic><topic>Chronic Disease</topic><topic>contraction</topic><topic>Diabetes</topic><topic>Diabetes Mellitus, Type 2 - complications</topic><topic>Diabetic Angiopathies - drug therapy</topic><topic>Diabetic Angiopathies - etiology</topic><topic>Diabetic Angiopathies - metabolism</topic><topic>diabète de type 2</topic><topic>Disease Models, Animal</topic><topic>GK rat</topic><topic>Health aspects</topic><topic>Indazoles - pharmacology</topic><topic>Kinases</topic><topic>Male</topic><topic>Nitric Oxide - metabolism</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>PDK1</topic><topic>Phenylephrine - pharmacology</topic><topic>Protein kinases</topic><topic>Pyrimidines - pharmacology</topic><topic>rat GK</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Rodents</topic><topic>Serotonin</topic><topic>Serotonin - pharmacology</topic><topic>Signal Transduction</topic><topic>Thromboxane A2 - analogs & derivatives</topic><topic>Type 2 diabetes</topic><topic>Vasoconstriction - drug effects</topic><topic>Vasoconstrictor Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Watanabe, Shun</creatorcontrib><creatorcontrib>Matsumoto, Takayuki</creatorcontrib><creatorcontrib>Taguchi, Kumiko</creatorcontrib><creatorcontrib>Kobayashi, Tsuneo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Canada</collection><collection>Science (Gale in Context)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Canadian journal of physiology and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Watanabe, Shun</au><au>Matsumoto, Takayuki</au><au>Taguchi, Kumiko</au><au>Kobayashi, Tsuneo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship between PDK1 and contraction in carotid arteries in Goto-Kakizaki rat, a spontaneous type 2 diabetic animal model</atitle><jtitle>Canadian journal of physiology and pharmacology</jtitle><addtitle>Can J Physiol Pharmacol</addtitle><date>2017-04-01</date><risdate>2017</risdate><volume>95</volume><issue>4</issue><spage>459</spage><epage>462</epage><pages>459-462</pages><issn>0008-4212</issn><eissn>1205-7541</eissn><coden>CJPPA3</coden><abstract>We studied the relationship between 3-phosphoinositide-dependent protein kinase 1 (PDK1) and contractions induced by serotonin, phenylephrine, and thromboxane A
2
mimetic (U46619) in the presence of nitric oxide synthase inhibitor in the carotid arteries of Goto-Kakizaki (GK) rats, a spontaneous type 2 diabetic model, in the chronic stage of disease. Serotonin-induced contraction was greater in the GK rats than in the control Wistar rats. A specific PDK1 inhibitor (GSK2334470) decreased the serotonin-induced contraction in the GK rats but not in the Wistar rats, and the difference in such contraction was abolished with this treatment. In GK rats, phenylephrine-induced contraction exhibited a leftward shift and U46619-induced contraction was greater still. Phenylephrine- and U46619-induced contractions were reduced by GSK2334470 in both groups. These results suggest, for the first time, that the contribution of PDK1 is different among 3 vasoconstrictors and that PDK1 contributed to increased serotonin-induced contraction in the carotid arteries of GK rats.</abstract><cop>Canada</cop><pub>NRC Research Press</pub><pmid>28118732</pmid><doi>10.1139/cjpp-2016-0372</doi><tpages>4</tpages></addata></record> |
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| ispartof | Canadian journal of physiology and pharmacology, 2017-04, Vol.95 (4), p.459-462 |
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| subjects | 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid - pharmacology 3-Phosphoinositide-Dependent Protein Kinases - antagonists & inhibitors 3-Phosphoinositide-Dependent Protein Kinases - metabolism Animals artère carotide Carotid arteries Carotid Arteries - drug effects Carotid Arteries - physiology carotid artery Chronic Disease contraction Diabetes Diabetes Mellitus, Type 2 - complications Diabetic Angiopathies - drug therapy Diabetic Angiopathies - etiology Diabetic Angiopathies - metabolism diabète de type 2 Disease Models, Animal GK rat Health aspects Indazoles - pharmacology Kinases Male Nitric Oxide - metabolism Nitric Oxide Synthase - antagonists & inhibitors PDK1 Phenylephrine - pharmacology Protein kinases Pyrimidines - pharmacology rat GK Rats Rats, Wistar Rodents Serotonin Serotonin - pharmacology Signal Transduction Thromboxane A2 - analogs & derivatives Type 2 diabetes Vasoconstriction - drug effects Vasoconstrictor Agents - pharmacology |
| title | Relationship between PDK1 and contraction in carotid arteries in Goto-Kakizaki rat, a spontaneous type 2 diabetic animal model |
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