Grass carp (Ctenopharyngodon idellus) invariant chain of the MHC class II chaperone protein associates with the class I molecule

The invariant chain (Ii) is an important immune molecule, as it assists major histocompatibility complex (MHC) class II molecules to present antigenic peptides. The relationship between the Ii and MHC molecules in teleosts remains poorly understood. This study focused on the molecular structure of g...

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Bibliographic Details
Published in:Fish & shellfish immunology 2017-04, Vol.63, p.1-8
Main Authors: Chen, Fang-fang, Lin, Hai-bin, Li, Jin-chun, Wang, Yong, Li, Juan, Zhang, Da-gan, Yu, Wei-yi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens, Differentiation, B-Lymphocyte - chemistry
Antigens, Differentiation, B-Lymphocyte - genetics
Antigens, Differentiation, B-Lymphocyte - metabolism
Association
Base Sequence
Carps - genetics
Carps - metabolism
Cloning, Molecular
Co-localization
DNA, Complementary - genetics
DNA, Complementary - metabolism
Female
Fish Proteins - chemistry
Fish Proteins - genetics
Fish Proteins - metabolism
Grass carp
Histocompatibility Antigens Class II - chemistry
Histocompatibility Antigens Class II - genetics
Histocompatibility Antigens Class II - metabolism
Invariant chain
Male
MHC
Molecular Chaperones - chemistry
Molecular Chaperones - genetics
Molecular Chaperones - metabolism
Organ Specificity
Phylogeny
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Alignment - veterinary
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Summary:The invariant chain (Ii) is an important immune molecule, as it assists major histocompatibility complex (MHC) class II molecules to present antigenic peptides. The relationship between the Ii and MHC molecules in teleosts remains poorly understood. This study focused on the molecular structure of grass carp Ii (gIi), its organ distribution, correlations with gene transcription, and the association with MHC. gIi cDNA was cloned using designed degenerate primers and the rapid amplification of cDNA ends method (RACE). The gIi sequence was 92%–96% similar to that of other teleosts, but only 52%–67% similar to that of mammals, respectively. The gIi gene was distributed in all 12 organs examined by PCR. The gIi gene transcription levels were markedly higher in organs enriched with immune cells than in other organs (P 
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2017.01.030