Cellulase with high β-glucosidase activity by Penicillium oxalicum under solid state fermentation and its use in hydrolysis of cassava residue

• Published in: World journal of microbiology & biotechnology 2017-02, Vol.33 (2), p.37-37, Article 37
• Main Authors: Su, Lin-Hui, Zhao, Shuai, Jiang, Sui-Xin, Liao, Xu-Zhong, Duan, Cheng-Jie, Feng, Jia-Xun
• Format: Article
• Language: English
• Subjects: Applied Microbiology; Bacterial Proteins - metabolism; beta-Glucosidase - metabolism; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cellulase - metabolism; Cellulose - chemistry; Environmental Engineering/Biotechnology; Fermentation; Glucose - chemistry; Hydrolysis; Life Sciences; Manihot - chemistry; Microbiology; Original Paper; Penicillium - enzymology; Penicillium - growth & development
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-016-2200-7

In this study, we investigated cellulase production by Penicillium oxalicum EU2106 under solid-state fermentation (SSF) and its hydrolysis efficiency toward NaOH–H 2 O 2 -pretreated cassava residue (NHCR) produced after bioethanol fermentation. Optimization of SSF cultivation conditions for P. oxalicum EU2106 using a Box-behnken design-based response-surface methodology resulted in maximal cellulase activity of 34.0 ± 2.8 filter-paper units/g dry substrate, exhibiting a ~ twofold increase relative to activities obtained under non-optimized conditions. Furthermore, SSF-derived cellulase converted 94.3 ± 1.5% of NHCR cellulose into glucose within 96 h. Interestingly, P. oxalicum EU2106 produced higher β-glucosidase activity under SSF conditions than that under submerged-state fermentation conditions, resulting in the elimination of cellobiose inhibition during the early stages of NHCR cellulose hydrolysis. Overall, this work provided an alternative for a potential cellulase source and a preferred option for cassava residue biotechnological application. Graphical Abstract