Prospective Study of CRMP4 Promoter Methylation in Prostate Biopsies as a Predictor For Lymph Node Metastases

• Published in: JNCI : Journal of the National Cancer Institute 2017-01, Vol.109 (6), p.djw282
• Main Authors: Gao, Xin, Li, Liao-Yuan, Rassler, Jörg, Pang, Jun, Chen, Ming-Kun, Liu, Wei-Peng, Chen, Zheng, Ren, Shan-Cheng, Zhou, Fang-Jian, Xie, Ke-Ji, Zhou, Xing, Qian, Hui-Jun, Bai, Xian-Zhong, Liu, Jiu-Min, Yang, Jiang-Gen, He, Dan, Shao, Chun-Kui, Su, Zu-Lan, Wang, Jing, Qiu, Jian-Guang, Ling, Li
• Format: Article
• Language: English
• Subjects: Aged; Area Under Curve; Biomarkers, Tumor - genetics; Biopsy; Case-Control Studies; CpG Islands; DNA Methylation; Humans; Lymphatic Metastasis; Male; Middle Aged; Muscle Proteins - genetics; Muscle Proteins - metabolism; Predictive Value of Tests; Promoter Regions, Genetic; Prospective Studies; Prostate - pathology; Prostatic Neoplasms - genetics; Prostatic Neoplasms - pathology; ROC Curve
• ISSN: 0027-8874; 1460-2105
• DOI: 10.1093/jnci/djw282

For patients with prostate cancer (PCa), the presence of pelvic lymph node metastasis (LNM) is a strong predictor of poor outcome. However, the approaches with promising sensitivity and specificity to detect LNM are still lacking. We investigated the value of collapsin response mediator protein 4 (CRMP4) promoter methylation in biopsies as a predictor for LNM. CRMP4 promoter methylation at two previously identified CpG sites was determined in 80 case-matched biopsy samples (the training set) using bisulfite pyrosequencing. The predictive cutoff value was independently validated using cohort I of 339 PCa patients (Southern China) and cohort II of 328 case patients (Germany, across China). Mann-Whitney U test, the receiver operating characteristic curve, McNemar's test, and logistic regression were used to assess data. All statistical tests were two-sided. In the training set, CRMP4 promoter methylation (≥15.0% methylated) was statistically significantly associated with LNM (P