Evaluation of the protective effect of pVAX-EtMIC3-recombined plasmid against E. tenella in chicken

• Published in: Parasitology research (1987) 2017-03, Vol.116 (3), p.1023-1028
• Main Authors: Wang, Xin-qiu, Wu, Lin-lin, Gao, Yan, Zhang, Yuan, Weng, Ya-Biao, Lin, Rui-Qing
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Antibodies, Protozoan - immunology; Biomedical and Life Sciences; Biomedicine; Body weight gain; Chickens - parasitology; Coccidians; Coccidiosis - immunology; Coccidiosis - parasitology; Coccidiosis - prevention & control; Coccidiosis - veterinary; Cytokines; DNA vaccines; Drug Evaluation; Economic aspects; Eimeria tenella - genetics; Eimeria tenella - immunology; Health aspects; Immune response; Immunization; Immunology; Infections; Medical Microbiology; Microbiology; Oocysts - immunology; Original Paper; Plasmids - genetics; Plasmids - metabolism; Poultry Diseases - immunology; Poultry Diseases - parasitology; Poultry Diseases - prevention & control; Protozoa; Protozoan Proteins - administration & dosage; Protozoan Proteins - genetics; Protozoan Proteins - immunology; Protozoan Vaccines - administration & dosage; Protozoan Vaccines - genetics; Protozoan Vaccines - immunology; Reorganization and restructuring; Vaccination; Vaccines; Vaccines, DNA - administration & dosage; Vaccines, DNA - genetics; Vaccines, DNA - immunology
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-017-5383-4

Coccidiosis caused by protozoan parasites of the genus Eimeria has a severe economic impact on commercial production worldwide. Micronemes of Eimeria play important roles in invading intestinal cell processes. In this study, the DNA vaccine expressing Eimeria tenella microneme protein 3 (EtMIC3) was constructed to evaluate its immune protective effect against E. tenella infection in chickens. The results demonstrated that chickens immunized with pVAX-EtMIC3 produced strong immune responses in the body, as shown by significant lymphocyte proliferation, cytokine production, and antibody responses. The average body weight gains of chickens in all the vaccinated groups were higher than those of non-vaccinated and challenged groups. In general, oocyst shedding was reduced, and bloody feces and gut lesion scores decreased. In addition, the survival rate of the immunized chickens increased compared to that of the unvaccinated and challenged control chickens. In summary, this study indicated that pVAX-EtMIC3 could induce protective immune effects against coccidiosis and that EtMIC3 is a potential vaccine candidate against coccidiosis.