Physicochemical Properties, Antioxidant and Anti‐proliferative Capacities of Dried Leaf and Its Extract from Xao tam phan (Paramignya trimera)

• Published in: Chemistry & biodiversity 2017-06, Vol.14 (6), p.n/a
• Main Authors: Nguyen, Van Tang, Sakoff, Jennette A., Scarlett, Christopher J.
• Format: Article
• Language: English
• Subjects: Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Antioxidant capaticity; Antioxidants; Antioxidants - isolation & purification; Antioxidants - pharmacology; Antiproliferatives; Anti‐proliferative potential; Bark; Berries; Biotechnology; Breast; Cancer; Catechin; Cell Line, Tumor; Colon; Ellagic acid; Enrichment; Extraction; Flavonoids - isolation & purification; Flavonoids - pharmacology; Freeze drying; Gallic acid; Gemcitabine; Glioblastoma; Harvesting; Humans; Lead compounds; Leaves; Lungs; Microwaves; Neuroblastoma; Pancreas; Pancreatic cancer; Paramignya trimera; Pharmaceutical industry; Phenolic compounds; Phenols; Phenols - isolation & purification; Phenols - pharmacology; Physicochemical properties; Physiochemistry; Phytochemicals; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Plants, Medicinal - chemistry; Proanthocyanidins - isolation & purification; Proanthocyanidins - pharmacology; Prostate; Protocatechuic acid; Quercetin; Rutaceae - chemistry; Rutin; Saponins - isolation & purification; Saponins - pharmacology; Shape memory alloys; Skin; Tumor cell lines; Xao tam phan
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.201600498

Xao tam phan (Paramignya trimera) has been used for the treatment of cancer and cancer‐like aliments. Among different parts of the P. trimera plant, leaf is considered as a residual part after harvesting of the root. This study aimed to determine the physiochemical properties and the antioxidant and anti‐proliferative capacities of P. trimera leaf (PTL) using microwave drying for the preparation of dry sample; MeOH and microwave‐assisted extraction for the preparation of crude extract; and freeze‐drying for the preparation of powdered extract. The results showed that total phenolic, total flavonoid, proanthocyanidin, and saponin contents of PTL prepared by microwave drying at 450 W were 25.4 mg gallic acid equiv. (GAE), 86.3 mg rutin equiv. (RE), 5.6 mg catechin equiv. (CE), and 702.1 mg escin equiv. (EE) per gram dried sample, respectively. Gallic acid, protocatechuic acid, ellagic acid, rutin, and quercetin were identified in the PTL MeOH extract. Dried PTL displayed potent antioxidant activity, while the powdered PTL extract exhibited great anti‐proliferative capacity on various cancer cell lines including MiaPaCa‐2 (pancreas), HT29 (colon), A2780 (ovarian), H460 (lung), A431 (skin), Du145 (prostate), BE2‐C (neuroblastoma), MCF‐7 (breast), MCF‐10A (normal breast), and U87, SJ‐G2, and SMA (glioblastoma). Anti‐proliferative capacity on pancreatic cancer cells (MiaCaPa2, BxPc3, and CFPAC1) of PTL extract (200 μg/ml) was significantly higher (P