High-level resistance to meropenem in clinical isolates of Pseudomonas aeruginosa in the absence of carbapenemases: role of active efflux and porin alterations

Highlights • P. aeruginosa from cystic fibrosis patients show high-level carbapenem resistance. • Meropenem was less active than imipenem against these isolates. • No carbapenemases were detected in these isolates. • Resistance mechanisms involved efflux overexpression and absence of OprD porin....

Full description

Saved in:
Bibliographic Details
Published in:International journal of antimicrobial agents 2016-12, Vol.48 (6), p.740-743
Main Authors: Chalhoub, Hussein, Sáenz, Yolanda, Rodriguez-Villalobos, Hector, Denis, Olivier, Kahl, Barbara C, Tulkens, Paul M, Van Bambeke, Françoise
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
beta-Lactamases - analysis
beta-Lactamases - genetics
Carbapenemase
Cystic fibrosis
Electrophoresis, Polyacrylamide Gel
Enzyme Inhibitors - metabolism
Gene Expression Profiling
Hospital-acquired pneumonia
Humans
Infectious Disease
MexAB–OprM
Microbial Sensitivity Tests
OprD
Pneumonia, Bacterial - microbiology
Polymerase Chain Reaction
Porins - genetics
Porins - metabolism
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - isolation & purification
Pseudomonas Infections - microbiology
Sequence Analysis, DNA
Thienamycins - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c498t-55abb8b89742b1a4158d0c400dea045dccbfc6b8463d1fa863a0872e1af99f903
cites cdi_FETCH-LOGICAL-c498t-55abb8b89742b1a4158d0c400dea045dccbfc6b8463d1fa863a0872e1af99f903
container_end_page 743
container_issue 6
container_start_page 740
container_title International journal of antimicrobial agents
container_volume 48
creator Chalhoub, Hussein
Sáenz, Yolanda
Rodriguez-Villalobos, Hector
Denis, Olivier
Kahl, Barbara C
Tulkens, Paul M
Van Bambeke, Françoise
description Highlights • P. aeruginosa from cystic fibrosis patients show high-level carbapenem resistance. • Meropenem was less active than imipenem against these isolates. • No carbapenemases were detected in these isolates. • Resistance mechanisms involved efflux overexpression and absence of OprD porin.
doi_str_mv 10.1016/j.ijantimicag.2016.09.012
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1862766976</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0924857916302849</els_id><sourcerecordid>1862766976</sourcerecordid><originalsourceid>FETCH-LOGICAL-c498t-55abb8b89742b1a4158d0c400dea045dccbfc6b8463d1fa863a0872e1af99f903</originalsourceid><addsrcrecordid>eNqNkk-LFDEQxRtR3HX1K0i8eekx6b-JB0EGdYUFBfUcqtPVsxnTyZhKD-6n8aua3tkV8eQpUPzeK_JeFcULwTeCi-7VfmP34JOdrYHdpsqjDVcbLqoHxbmQfVX2StQPi3OuqqaUba_OiidEe85FWzft4-KskqKSXPXnxa9Lu7suHR7RsYhkKYE3yFJgM8ZwQI8zs54ZZ31e5pil4CAhsTCxz4TLGObggRhgXHbWB4IVT9fIYCBcrTJoIA5w6wWE9JrF4G7nYJI9IsNpcstPBn5khxCzHFzCCMkGT0-LRxM4wmd370Xx7f27r9vL8urTh4_bt1elaZRMZdvCMMhBqr6pBgGNaOXITcP5iMCbdjRmmEw3yKarRzGB7GrgOSgUMCk1KV5fFC9PvocYfixISc-WDDoHHsNCWsiu6rtO9V1G1Qk1MRBFnPQh2hnijRZcr_3ovf6rH732o7nSuZ-sfX63ZhlmHP8o7wvJwPYEYP7s0WLUZOya42gjmqTHYP9rzZt_XO4L_I43SPuwRJ_T1EJTpbn-sh7Keieiq3klG1X_BjOfwHE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1862766976</pqid></control><display><type>article</type><title>High-level resistance to meropenem in clinical isolates of Pseudomonas aeruginosa in the absence of carbapenemases: role of active efflux and porin alterations</title><source>ScienceDirect Freedom Collection</source><creator>Chalhoub, Hussein ; Sáenz, Yolanda ; Rodriguez-Villalobos, Hector ; Denis, Olivier ; Kahl, Barbara C ; Tulkens, Paul M ; Van Bambeke, Françoise</creator><creatorcontrib>Chalhoub, Hussein ; Sáenz, Yolanda ; Rodriguez-Villalobos, Hector ; Denis, Olivier ; Kahl, Barbara C ; Tulkens, Paul M ; Van Bambeke, Françoise</creatorcontrib><description>Highlights • P. aeruginosa from cystic fibrosis patients show high-level carbapenem resistance. • Meropenem was less active than imipenem against these isolates. • No carbapenemases were detected in these isolates. • Resistance mechanisms involved efflux overexpression and absence of OprD porin.</description><identifier>ISSN: 0924-8579</identifier><identifier>EISSN: 1872-7913</identifier><identifier>DOI: 10.1016/j.ijantimicag.2016.09.012</identifier><identifier>PMID: 28128097</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Anti-Bacterial Agents - pharmacology ; beta-Lactamases - analysis ; beta-Lactamases - genetics ; Carbapenemase ; Cystic fibrosis ; Electrophoresis, Polyacrylamide Gel ; Enzyme Inhibitors - metabolism ; Gene Expression Profiling ; Hospital-acquired pneumonia ; Humans ; Infectious Disease ; MexAB–OprM ; Microbial Sensitivity Tests ; OprD ; Pneumonia, Bacterial - microbiology ; Polymerase Chain Reaction ; Porins - genetics ; Porins - metabolism ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - isolation &amp; purification ; Pseudomonas Infections - microbiology ; Sequence Analysis, DNA ; Thienamycins - pharmacology</subject><ispartof>International journal of antimicrobial agents, 2016-12, Vol.48 (6), p.740-743</ispartof><rights>2016 Elsevier B.V. and International Society of Chemotherapy</rights><rights>Copyright © 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-55abb8b89742b1a4158d0c400dea045dccbfc6b8463d1fa863a0872e1af99f903</citedby><cites>FETCH-LOGICAL-c498t-55abb8b89742b1a4158d0c400dea045dccbfc6b8463d1fa863a0872e1af99f903</cites><orcidid>0000-0003-3153-5369 ; 0000-0002-7121-3623 ; 0000-0002-0052-7991 ; 0000-0003-3041-1591</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28128097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chalhoub, Hussein</creatorcontrib><creatorcontrib>Sáenz, Yolanda</creatorcontrib><creatorcontrib>Rodriguez-Villalobos, Hector</creatorcontrib><creatorcontrib>Denis, Olivier</creatorcontrib><creatorcontrib>Kahl, Barbara C</creatorcontrib><creatorcontrib>Tulkens, Paul M</creatorcontrib><creatorcontrib>Van Bambeke, Françoise</creatorcontrib><title>High-level resistance to meropenem in clinical isolates of Pseudomonas aeruginosa in the absence of carbapenemases: role of active efflux and porin alterations</title><title>International journal of antimicrobial agents</title><addtitle>Int J Antimicrob Agents</addtitle><description>Highlights • P. aeruginosa from cystic fibrosis patients show high-level carbapenem resistance. • Meropenem was less active than imipenem against these isolates. • No carbapenemases were detected in these isolates. • Resistance mechanisms involved efflux overexpression and absence of OprD porin.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>beta-Lactamases - analysis</subject><subject>beta-Lactamases - genetics</subject><subject>Carbapenemase</subject><subject>Cystic fibrosis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme Inhibitors - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Hospital-acquired pneumonia</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>MexAB–OprM</subject><subject>Microbial Sensitivity Tests</subject><subject>OprD</subject><subject>Pneumonia, Bacterial - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>Porins - genetics</subject><subject>Porins - metabolism</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - isolation &amp; purification</subject><subject>Pseudomonas Infections - microbiology</subject><subject>Sequence Analysis, DNA</subject><subject>Thienamycins - pharmacology</subject><issn>0924-8579</issn><issn>1872-7913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNkk-LFDEQxRtR3HX1K0i8eekx6b-JB0EGdYUFBfUcqtPVsxnTyZhKD-6n8aua3tkV8eQpUPzeK_JeFcULwTeCi-7VfmP34JOdrYHdpsqjDVcbLqoHxbmQfVX2StQPi3OuqqaUba_OiidEe85FWzft4-KskqKSXPXnxa9Lu7suHR7RsYhkKYE3yFJgM8ZwQI8zs54ZZ31e5pil4CAhsTCxz4TLGObggRhgXHbWB4IVT9fIYCBcrTJoIA5w6wWE9JrF4G7nYJI9IsNpcstPBn5khxCzHFzCCMkGT0-LRxM4wmd370Xx7f27r9vL8urTh4_bt1elaZRMZdvCMMhBqr6pBgGNaOXITcP5iMCbdjRmmEw3yKarRzGB7GrgOSgUMCk1KV5fFC9PvocYfixISc-WDDoHHsNCWsiu6rtO9V1G1Qk1MRBFnPQh2hnijRZcr_3ovf6rH732o7nSuZ-sfX63ZhlmHP8o7wvJwPYEYP7s0WLUZOya42gjmqTHYP9rzZt_XO4L_I43SPuwRJ_T1EJTpbn-sh7Keieiq3klG1X_BjOfwHE</recordid><startdate>20161201</startdate><enddate>20161201</enddate><creator>Chalhoub, Hussein</creator><creator>Sáenz, Yolanda</creator><creator>Rodriguez-Villalobos, Hector</creator><creator>Denis, Olivier</creator><creator>Kahl, Barbara C</creator><creator>Tulkens, Paul M</creator><creator>Van Bambeke, Françoise</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3153-5369</orcidid><orcidid>https://orcid.org/0000-0002-7121-3623</orcidid><orcidid>https://orcid.org/0000-0002-0052-7991</orcidid><orcidid>https://orcid.org/0000-0003-3041-1591</orcidid></search><sort><creationdate>20161201</creationdate><title>High-level resistance to meropenem in clinical isolates of Pseudomonas aeruginosa in the absence of carbapenemases: role of active efflux and porin alterations</title><author>Chalhoub, Hussein ; Sáenz, Yolanda ; Rodriguez-Villalobos, Hector ; Denis, Olivier ; Kahl, Barbara C ; Tulkens, Paul M ; Van Bambeke, Françoise</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-55abb8b89742b1a4158d0c400dea045dccbfc6b8463d1fa863a0872e1af99f903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>beta-Lactamases - analysis</topic><topic>beta-Lactamases - genetics</topic><topic>Carbapenemase</topic><topic>Cystic fibrosis</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzyme Inhibitors - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Hospital-acquired pneumonia</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>MexAB–OprM</topic><topic>Microbial Sensitivity Tests</topic><topic>OprD</topic><topic>Pneumonia, Bacterial - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>Porins - genetics</topic><topic>Porins - metabolism</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - isolation &amp; purification</topic><topic>Pseudomonas Infections - microbiology</topic><topic>Sequence Analysis, DNA</topic><topic>Thienamycins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chalhoub, Hussein</creatorcontrib><creatorcontrib>Sáenz, Yolanda</creatorcontrib><creatorcontrib>Rodriguez-Villalobos, Hector</creatorcontrib><creatorcontrib>Denis, Olivier</creatorcontrib><creatorcontrib>Kahl, Barbara C</creatorcontrib><creatorcontrib>Tulkens, Paul M</creatorcontrib><creatorcontrib>Van Bambeke, Françoise</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of antimicrobial agents</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chalhoub, Hussein</au><au>Sáenz, Yolanda</au><au>Rodriguez-Villalobos, Hector</au><au>Denis, Olivier</au><au>Kahl, Barbara C</au><au>Tulkens, Paul M</au><au>Van Bambeke, Françoise</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-level resistance to meropenem in clinical isolates of Pseudomonas aeruginosa in the absence of carbapenemases: role of active efflux and porin alterations</atitle><jtitle>International journal of antimicrobial agents</jtitle><addtitle>Int J Antimicrob Agents</addtitle><date>2016-12-01</date><risdate>2016</risdate><volume>48</volume><issue>6</issue><spage>740</spage><epage>743</epage><pages>740-743</pages><issn>0924-8579</issn><eissn>1872-7913</eissn><abstract>Highlights • P. aeruginosa from cystic fibrosis patients show high-level carbapenem resistance. • Meropenem was less active than imipenem against these isolates. • No carbapenemases were detected in these isolates. • Resistance mechanisms involved efflux overexpression and absence of OprD porin.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28128097</pmid><doi>10.1016/j.ijantimicag.2016.09.012</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-3153-5369</orcidid><orcidid>https://orcid.org/0000-0002-7121-3623</orcidid><orcidid>https://orcid.org/0000-0002-0052-7991</orcidid><orcidid>https://orcid.org/0000-0003-3041-1591</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0924-8579
ispartof International journal of antimicrobial agents, 2016-12, Vol.48 (6), p.740-743
issn 0924-8579
1872-7913
language eng
recordid cdi_proquest_miscellaneous_1862766976
source ScienceDirect Freedom Collection
subjects Anti-Bacterial Agents - pharmacology
beta-Lactamases - analysis
beta-Lactamases - genetics
Carbapenemase
Cystic fibrosis
Electrophoresis, Polyacrylamide Gel
Enzyme Inhibitors - metabolism
Gene Expression Profiling
Hospital-acquired pneumonia
Humans
Infectious Disease
MexAB–OprM
Microbial Sensitivity Tests
OprD
Pneumonia, Bacterial - microbiology
Polymerase Chain Reaction
Porins - genetics
Porins - metabolism
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - isolation & purification
Pseudomonas Infections - microbiology
Sequence Analysis, DNA
Thienamycins - pharmacology
title High-level resistance to meropenem in clinical isolates of Pseudomonas aeruginosa in the absence of carbapenemases: role of active efflux and porin alterations
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T07%3A32%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High-level%20resistance%20to%20meropenem%20in%20clinical%20isolates%20of%20Pseudomonas%20aeruginosa%20in%20the%20absence%20of%20carbapenemases:%20role%20of%20active%20efflux%20and%20porin%20alterations&rft.jtitle=International%20journal%20of%20antimicrobial%20agents&rft.au=Chalhoub,%20Hussein&rft.date=2016-12-01&rft.volume=48&rft.issue=6&rft.spage=740&rft.epage=743&rft.pages=740-743&rft.issn=0924-8579&rft.eissn=1872-7913&rft_id=info:doi/10.1016/j.ijantimicag.2016.09.012&rft_dat=%3Cproquest_cross%3E1862766976%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c498t-55abb8b89742b1a4158d0c400dea045dccbfc6b8463d1fa863a0872e1af99f903%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1862766976&rft_id=info:pmid/28128097&rfr_iscdi=true