Identification of olive-tree cultivars with SCAR markers

There is an urgent need for the developmentof early identification techniques inolive-trees due to the economic importanceof cultivar identification in periods ofexpansion like now. We have been able toidentify 22 olive-tree cultivars using only10 different, specific, repeatable markers.These marker...

Full description

Saved in:
Bibliographic Details
Published in:Euphytica 2003, Vol.129 (1), p.33-41
Main Authors: BAUTISTA, Rocio, CRESPILLO, Remedios, CANOVAS, Francisco M, GONZALO CLAROS, M
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Cultivars
Cultivars: description, identification, tests, catalogs
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
Varietal selection. Specialized plant breeding, plant breeding aims
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:There is an urgent need for the developmentof early identification techniques inolive-trees due to the economic importanceof cultivar identification in periods ofexpansion like now. We have been able toidentify 22 olive-tree cultivars using only10 different, specific, repeatable markers.These markers were designed by the cloningof significant RAPD bands obtained in PCRperformed on bulked DNA to retain thegenetic variability of each cultivar.Clones were partially or totally sequencedand new primers derived from thesesequences were used to obtain SequenceCharacterised Amplified Region (SCAR)fragments. We have demonstrated that theuse of the 10 SCAR markers is enough toprovide a simple, cheap, and reliableprocedure to identify 22 geographicallyrelated olive-tree cultivars.[PUBLICATION ABSTRACT]
ISSN:0014-2336
1573-5060
DOI:10.1023/A:1021528122049