High-virulence CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from commercial turkeys

Abstract This study reports the high-virulence phylogenetic backgrounds of CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from turkeys sent to slaughter and condemned by airsacculitis in Brazil. Among 300 air sac samples, seven E. coli strains produced plasmid-mediat...

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Published in:Diagnostic microbiology and infectious disease 2017-01, Vol.87 (1), p.64-67
Main Authors: da Silva, Ketrin Cristina, Cunha, Marcos Paulo Vieira, Cerdeira, Louise, de Oliveira, Maria Gabriela Xavier, de Oliveira, Mirela Caroline Vilela, Gomes, Cleise Ribeiro, Lincopan, Nilton, Knöbl, Terezinha, Moreno, Andrea Micke
Format: Article
Language:English
Subjects:
Abattoirs
Air Sacs - microbiology
Animals
beta-Lactamases - analysis
beta-Lactamases - genetics
Brazil
CMY-2
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
ESBL
Escherichia coli
Escherichia coli - classification
Escherichia coli - enzymology
Escherichia coli - isolation & purification
Escherichia coli - pathogenicity
Escherichia coli Proteins - analysis
Escherichia coli Proteins - genetics
Genotype
IncI1/ST12
Infectious Disease
Internal Medicine
Plasmid
Plasmids - analysis
Plasmids - classification
Salmonella enterica
Sequence Analysis, DNA
Turkey
Turkeys - microbiology
Virulence
Virulence Factors - analysis
Virulence Factors - genetics
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Summary:Abstract This study reports the high-virulence phylogenetic backgrounds of CMY-2- and CTX-M-2-producing avian pathogenic Escherichia coli strains isolated from turkeys sent to slaughter and condemned by airsacculitis in Brazil. Among 300 air sac samples, seven E. coli strains produced plasmid-mediated CMY-2-type AmpC, of which three carried also the blaCTX-M-2 Extended Spectrum Beta-Lactamase encoding gene. Interestingly, the transfer of the blaCMY-2 gene was positive for three E. coli strains, being associated with the presence of IncI1 plasmids. The complete sequence of the representative pJB10 plasmid revealed that the blaCMY-2 gene was within a transposon-like element in the classical genetic environment consisting of tnpA - blaCMY-2 - blc - sugE structure. This plasmid with 94-kb belonged to the sequence type (ST) 12 among IncI1 plasmids, which has been associated with the worldwide spread of blaCMY-2 among Salmonella enterica and E. coli . Furthermore, to the best of our knowledge, this is the first complete sequence of a CMY-2-encoding plasmid derived from an Escherichia coli isolated from food-producing animals in Latin America.
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2016.10.001