Comparative evaluation of aggressiveness traits in staphylococcal strains from severe infections versus nasopharyngeal carriage

Despite their commensal status, staphylococci can become problematic pathogens expressing multiple and redundant virulence factors. This study aimed to evaluate aggressiveness markers comparatively in staphylococcal strains isolated from severe infections versus asymptomatic carriage in order to ide...

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Bibliographic Details
Published in:Microbial pathogenesis 2017-01, Vol.102, p.45-53
Main Authors: Săndulescu, Oana, Bleotu, Coralia, Matei, Lilia, Streinu-Cercel, Anca, Oprea, Mihaela, Drăgulescu, Elena Carmina, Chifiriuc, Mariana Carmen, Rafila, Alexandru, Pirici, Daniel, Tălăpan, Daniela, Dorobăţ, Olga Mihaela, Neguţ, Alina Cristina, Oţelea, Dan, Berciu, Ioana, Ion, Daniela Adriana, Codiţă, Irina, Calistru, Petre Iacob, Streinu-Cercel, Adrian
Format: Article
Language:English
Subjects:
Adhesion
Adolescent
Adult
Aged
Aged, 80 and over
Anti-Bacterial Agents - pharmacology
Bacterial Adhesion
Biofilm
Biofilms
Carrier State
Cell Line
Child
Child, Preschool
Comorbidity
Female
Genetic Variation
Humans
Infant
Infant, Newborn
Invasion
Male
Microbial Sensitivity Tests
Middle Aged
Nasopharynx - microbiology
Quantitative Trait, Heritable
S. aureus
Severity of Illness Index
Soluble virulence factors
Staphylococcal Infections - diagnosis
Staphylococcal Infections - microbiology
Staphylococcus
Staphylococcus - classification
Staphylococcus - drug effects
Staphylococcus - pathogenicity
Staphylococcus - physiology
Virulence
Virulence Factors
Young Adult
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Summary:Despite their commensal status, staphylococci can become problematic pathogens expressing multiple and redundant virulence factors. This study aimed to evaluate aggressiveness markers comparatively in staphylococcal strains isolated from severe infections versus asymptomatic carriage in order to identify clinically relevant bacterial traits that could easily be detected in clinical practice and could be suggestive for particular host-pathogen interactions such as cyto-adhesion or biofilm formation, ultimately orienting the clinical decision-making process. We have used in vitro phenotypic methods to assess adhesion to and invasion of eukaryotic cells, biofilm development, and expression of soluble virulence factors in 92 Staphylococcus spp. strains. The adhesion index, invasion capacity, biofilm formation and expression of soluble factors did not differ significantly between clinical and commensal strains. The major bacterial traits we found to be significantly more prevalent in clinical staphylococci were the aggregative adhesion pattern (P = 0.012), cluster adhesion (P = 0.001) and tetrad morphology (P = 0.018). The aggregative adhesion pattern was correlated with higher cyto-adhesion (P 
ISSN:0882-4010
1096-1208
DOI:10.1016/j.micpath.2016.11.006