Molecular characterization of a single-chain antibody variable fragment (scFv) specific for PspA from Streptococcus pneumoniae

• Published in: Biochemical and biophysical research communications 2017-01, Vol.482 (1), p.141-146
• Main Authors: Jang, ShinA, Kim, Gyuhee, Oh, Jihye, Lee, Seungyeop, Kim, Dongho, Kim, Kook-Han, Kim, Yong Ho, Rhee, Dong-Kwon, Lee, Sangho
• Format: Article
• Language: English
• Subjects: 2B11; Bacterial Proteins - chemistry; Bacterial Proteins - immunology; Bacterial Proteins - isolation & purification; Binding Sites; Biosensing Techniques; Epitope; Immunoassay; Molecular Docking Simulation; Phage display; Protein Binding; PspA; Single-Chain Antibodies - chemistry; Single-Chain Antibodies - immunology; Single-Chain Antibodies - isolation & purification; Single-chain antibody variable fragment; Streptococcus pneumoniae; Streptococcus pneumoniae - chemistry; Streptococcus pneumoniae - immunology
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2016.10.150

Streptococcus pneumoniae is a major infectious agent responsible for pneumonia, otitis media, sepsis and meningitis. Pneumococcal surface protein A (PspA) is a well-characterized virulence factor localized on the surface and a target for vaccine development. In this study, we screened a single-chain antibody variable fragment (scFv) using phage display from a human synthetic library to select a clone 2B11. Affinity (Kd) of 2B11 was measured to be 5 nM using biolayer interferometry. 2B11 exhibited a dose-dependent recognition of recombinant PspA with no cross-reactivity towards pneumococcal antigens. The epitope on PspA was defined to residues 231–242 by mutational analysis. Molecular docking analysis supported the experimentally determined epitope, suggesting that the helix spanning residues 231–242 can bind to 2B11 with residues in the CDR-H3 (complementarity determining region 3 in the heavy chain) actively participating in the molecular contacts. Comparison of 2B11 with a commercial PspA antibody revealed that 2B11 exhibited a better specificity towards recombinant PspA antigen. 2B11 was capable of detecting endogenous PspA from pneumococcal lysates with affinity similar to that of the commercial antibody. Our study provides a molecular tool for biosensors detecting pneumococcal diseases. •A single-chain antibody variable fragment specific to pneumococcal PspA was selected.•Binding affinity of the antibody fragment, 2B11, was determined to be 5 nM.•Epitope on PspA for 2B11 was determined and supported by docking analysis.•2B11 was capable of detecting PspA from pneumococcal lysates.