Comparative study on characterization and wound healing potential of goat (Capra hircus) mesenchymal stem cells derived from fetal origin amniotic fluid and adult bone marrow

Caprine amniotic fluid (cAF) and bone marrow cells (cBM) were isolated, expanded and phenotypically characterized by mesenchymal stem cells (MSCs) specific cell surface markers. Both cell types were compared for multilineage differentiation potential by flow cytometry using specific antibodies again...

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Bibliographic Details
Published in:Research in veterinary science 2017-06, Vol.112, p.81-88
Main Authors: Pratheesh, M.D., Dubey, Pawan K., Gade, Nitin E., Nath, Amar, Sivanarayanan, T.B., Madhu, D.N., Somal, Anjali, Baiju, Indu, Sreekumar, T.R., Gleeja, V.L., Bhatt, Irfan A., Chandra, Vikash, Amarpal, Sharma, Bhaskar, Saikumar, G., Taru Sharma, G.
Format: Article
Language:English
Subjects:
Amniotic fluid
Amniotic Fluid - cytology
Animals
Antibodies
Biomarkers
Bone marrow
Bone Marrow Cells - cytology
Caprine
Cardiomyocytes
Cell cycle
Cell Differentiation
Cell surface
Collagen
Comparative studies
Cytometry
Differentiation
Fetuses
Flow cytometry
Goats
Injection
Mesenchymal stem cells
Mesenchymal Stromal Cells - physiology
Mesenchyme
Penicillin
Rabbits
Random Allocation
Skin
Stem Cell Transplantation - veterinary
Stem cells
Surface markers
Transforming growth factor-b1
Vascular endothelial growth factor
Vascularization
Veterinary medicine
Wound Healing
Wounds and Injuries - therapy
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Summary:Caprine amniotic fluid (cAF) and bone marrow cells (cBM) were isolated, expanded and phenotypically characterized by mesenchymal stem cells (MSCs) specific cell surface markers. Both cell types were compared for multilineage differentiation potential by flow cytometry using specific antibodies against lineage specific markers. Furthermore, in vitro expanded cAF-MSCs showed higher expression of trophic factors viz. VEGF and TGF-β1 as compared to cBM-MSCs. Full-skin thickness excisional wounds created on either side of the dorsal midline (thoracolumbar) of New Zealand White rabbits were randomly assigned to subcutaneous injection of either fetal origin cAF-MSCs (n=4) or adult cBM-MSCs (n=4) or sterile PBS (control, n=4). The rate of wound closure was found faster (p
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2016.12.009