Oxidized low‐density lipoprotein, lipid and calcium aggregates reveal oxidative stress and inflammation in the conjunctiva of glaucoma patients

Purpose Conjunctival specimens from primary open‐angle glaucoma (POAG), exfoliation glaucoma (ExG) patients and controls were histologically analysed for oxidized low‐density lipoprotein (ox‐LDL), lipid and calcium aggregates. Our goal was to use them as biomarkers of oxidative stress and inflammati...

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Published in:Acta ophthalmologica (Oxford, England) England), 2017-06, Vol.95 (4), p.378-385
Main Authors: Helin‐Toiviainen, Minna, Rönkkö, Seppo, Kaarniranta, Kai, Puustjärvi, Tuomo, Rekonen, Petri, Ollikainen, Minna, Uusitalo, Hannu
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Aggregates
Alizarin
Biomarkers - metabolism
Calcium
Calcium - metabolism
Conjunctiva
Conjunctiva - metabolism
deep sclerectomy
exfoliation glaucoma
Exfoliation Syndrome - diagnosis
Exfoliation Syndrome - metabolism
Exfoliation Syndrome - physiopathology
Female
Glaucoma
Glaucoma, Open-Angle - diagnosis
Glaucoma, Open-Angle - metabolism
Glaucoma, Open-Angle - physiopathology
Humans
Immunohistochemistry
Inflammation
Intraocular Pressure
Lipids
Lipids - analysis
Lipoproteins, LDL - metabolism
Low density lipoprotein
Male
Middle Aged
Oxidative Stress
primary open‐angle glaucoma
Retina
Scars
Strabismus
Stroma
Surgery
Wound healing
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Summary:Purpose Conjunctival specimens from primary open‐angle glaucoma (POAG), exfoliation glaucoma (ExG) patients and controls were histologically analysed for oxidized low‐density lipoprotein (ox‐LDL), lipid and calcium aggregates. Our goal was to use them as biomarkers of oxidative stress and inflammation and to evaluate their correlation with glaucoma and impact on surgical outcome. Methods Conjunctival samples were obtained from POAG (n = 14) and ExG (n = 17) patients and from control subjects (n = 11) operated for macular hole, retinal detachment or strabismus. Immunohistochemistry was performed using the antibody against ox‐LDL. Lipids and calcium were analysed by histochemical stainings with Nile red and Alizarin red S, respectively. Results Immunoreaction for ox‐LDL was significantly increased in POAG (p = 0.049) and the number of lipid aggregates was significantly higher in ExG (p = 0.009) when compared to control. When POAG and ExG patients were grouped according to the outcome of deep sclerectomy (DS) surgery, the number of lipid (p 
ISSN:1755-375X
1755-3768
DOI:10.1111/aos.13380