REPLY TO COMMENT ON THE LIFE CYCLE AND TOXICITY OF PFIESTERIA PISCICIDA REVISITED

• Published in: Journal of phycology 2002-12, Vol.38 (6), p.1268-1272
• Main Authors: Litaker, R. Wayne, Vandersea, Mark W., Kibler, Steven R., Noga, Edward J., Tester, Patricia A.
• Format: Article
• Language: English
• Subjects: amoeba; chrysophytes; dinoflagellate; division cyst; life cycle; meiosis; Pfiesteria; Pfiesteria piscicida; sexual reproduction
• ISSN: 0022-3646; 1529-8817
• DOI: 10.1046/j.1529-8817.2002.02133.x

Free-living, marine dinoflagellates are typified by a well-defined, haplontic life cycle with relatively few stages. The most unusual departure from this life cycle is one reported for the heterotrophic dinoflagellate Pfiesteria piscicida Steidinger et Burkholder. This species is alleged to have at least 24 life cycle stages including amoebae and a chrysophyte-like cyst form (Burkholder et al. 1992, Burkholder and Glasgow 1997a) not previously known in free-living marine dinoflagellates. Litaker et al. (2002) redescribed the life cycle of P. piscicida from single-cell isolates and found only life cycle stages typical of free-living marine dinoflagellates. The discrepancy between these observations and the life cycle reported in the literature prompted a rigorous study to resolve the life cycle of P. piscicida.Burkholder and Glasgow (2002) took exception to this study, arguing that Litaker et al. (2002) misunderstood the life cycle of P. piscicida and ignored recent publications. We present a rebuttal of their criticisms and suggest a simple way to resolve the discrepancies in the P. piscicida life cycle.