Growth and release of hydroxycinnamic acids from Brewer’s spent grain by Streptomyces avermitilis CECT 3339

• Published in: Enzyme and microbial technology 2003-01, Vol.32 (1), p.140-144
• Main Authors: Bartolomé, Begoña, Gómez-Cordovés, Carmen, Sancho, Ana I, Dı́ez, Nardy, Ferreira, Patricia, Soliveri, Juan, Copa-Patiño, José L
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biotechnology; Brewer’s spent grain; Ferulic acid; Feruloyl esterase; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Streptomycetes; Xylanases
• ISSN: 0141-0229; 1879-0909
• DOI: 10.1016/S0141-0229(02)00277-6

Streptomyces avermitilis CECT 3339 is grown on Brewer’s spent grain (BSG) and the production of feruloyl esterase (FAE) and (1→4)-β- d-xylan xilanohydrolase (xylanase) activities is studied over 5 days. Maximum level of xylanases was found at day 1. FAE activity on methyl ferulate reached a maximum level at day 2, whereas FAE activity on feruloylated oligosaccharides, either from wheat bran or sugar-beet pulp, was maximal at day 1. The cultures (1–5 days) from S. avermilitis CECT 3339 grown on BSG and on other two agro-industrial residues such as de-starched wheat bran and sugar-beet pulp were tested for the release of hydroxycinnamic acids (ferulic and p-coumaric acids) from BSG. Most ferulic acid (FA) was released when culture supernatants from day 1 and BSG as carbon source (43% of total alkali-extractable ferulic acid) and from day 2 and de-starched wheat bran as carbon source (41.2%) were used. The level of p-coumaric released in all cases was lower (