On-line biomass monitoring in bench-scale stirred bioreactors using parts of a liquid chromatography system

A cell density monitor, comprising parts of a liquid chromatography system, was adapted for on-line real-time measurement of bacterial cell density up to 75 g l^sup -1^. The device dilutes the sample containing cells and measures the absorbancy at 280 nm. This detector gives an excellent correlation...

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Bibliographic Details
Published in:Biotechnology letters 2002-12, Vol.24 (23), p.1999-2003
Main Authors: FORCATO, Diego O, PECORA, Rolando P, KIVATINITZ, Silvia C
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Bioreactors
Biotechnology
Fermentation
Fundamental and applied biological sciences. Psychology
Liquid chromatography
Methods. Procedures. Technologies
Various methods and equipments
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Description
Summary:A cell density monitor, comprising parts of a liquid chromatography system, was adapted for on-line real-time measurement of bacterial cell density up to 75 g l^sup -1^. The device dilutes the sample containing cells and measures the absorbancy at 280 nm. This detector gives an excellent correlation with off-line measurements, good reproducibility, a low limit of detection (3.5 mg l^sup -1^), and becomes a low cost alternative for fermentation monitoring in small stirred bioreactors.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1021110730741