Fluorescent Probe Based on Azobenzene-Cyclopalladium for the Selective Imaging of Endogenous Carbon Monoxide under Hypoxia Conditions

Carbon monoxide (CO), a crucial gas message molecule, plays an important role in the regulation of physiological and pathological process. Hypoxia-induced CO is involved in modulating various cellular activities, including signal transduction, proliferation, and apoptosis. However, tracking CO fluct...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-11, Vol.88 (22), p.11154-11159
Main Authors: Li, Yong, Wang, Xu, Yang, Jie, Xie, Xilei, Li, Mengmeng, Niu, Jinye, Tong, Lili, Tang, Bo
Format: Article
Language:English
Subjects:
Analytical chemistry
Apoptosis
Carbon monoxide
Cells
Chemical compounds
Fluorescence
Hypoxia
Imaging
Palladium
Signal transduction
Synthesis (chemistry)
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Summary:Carbon monoxide (CO), a crucial gas message molecule, plays an important role in the regulation of physiological and pathological process. Hypoxia-induced CO is involved in modulating various cellular activities, including signal transduction, proliferation, and apoptosis. However, tracking CO fluctuation in the hypoxic cells is still a challenge due to lack of straightforward, visualized, and noninvasive tools. In this work, based on metal palladium-catalyzed reaction, we present the rational design, synthesis, and biological utility of an azobenzene-cyclopalladium-based fluorescent probe, ACP-2, for CO monitoring. ACP-2 exhibits capacity of detecting CO in aqueous buffer solution and live cells with high sensitivity and specificity. Utilizing ACP-2, we displayed a direct and visual evidence of endogenous CO up-regulation in live cells induced by hypoxia. Moreover, CO up-regulation during oxygen-glucose deprivation/reperfusion (OGD/R) was also imaged and certified by ACP-2.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.6b03376