Biostable L‑DNA-Templated Aptamer-Silver Nanoclusters for Cell-Type-Specific Imaging at Physiological Temperature

The high susceptibility of the natural D-conformation of DNA (D-DNA) to nucleases greatly limits the application of DNA-templated silver nanoclusters (Ag NCs) in biological matrixes. Here we demonstrate that the L-conformation of DNA (L-DNA), the enantiomer of D-DNA, can also be used for the prepara...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-11, Vol.88 (22), p.10800-10804
Main Authors: Han, Gui-Mei, Jia, Zhen-Zhen, Zhu, Yan-Jun, Jiao, Jia-Jia, Kong, De-Ming, Feng, Xi-Zeng
Format: Article
Language:English
Subjects:
Biochemistry
Biocompatibility
Deoxyribonucleic acid
Digestion
DNA
Enantiomers
Imaging
Molecules
Nanoscience
Nanostructure
Nuclease
Physiology
Scientific imaging
Silver
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Summary:The high susceptibility of the natural D-conformation of DNA (D-DNA) to nucleases greatly limits the application of DNA-templated silver nanoclusters (Ag NCs) in biological matrixes. Here we demonstrate that the L-conformation of DNA (L-DNA), the enantiomer of D-DNA, can also be used for the preparation of aptamer-Ag NCs. The extraordinary resistance of L-DNA to nuclease digestion confers much higher biostability to these NCs than those templated by D-DNA, thus making cell-type-specific imaging possible at physiological temperatures, using at least 100-times lower Ag NC concentration than reported D-DNA-templated ones. The L-DNA-templated metal NC probes with enhanced biostability might promote the applications of metal nanocluster probes in complex biological systems.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.6b02871