Polarization sensitive optical coherence microscopy forbrain imaging

Optical coherence tomography (OCT) and optical coherence microscopy(OCM) have demonstrated the ability to investigate cyto- andmyelo-architecture in the brain. Polarization-sensitive OCT providessensitivity to additional contrast mechanisms, specifically thebirefringence of myelination and, therefor...

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Bibliographic Details
Published in:Optics letters 2016-05, Vol.41 (10), p.2213-2216
Main Authors: Wang, Hui, Akkin, Taner, Magnain, Caroline, Wang, Ruopeng, Dubb, Jay, Kostis, William J, Yaseen, Mohammad A, Cramer, Avilash, Sakadzic, Sava, Boas, David
Format: Article
Language:English
Subjects:
Brain
Coherence
Fibers
Microscopes
Microscopy
Optical Coherence Tomography
Optical fibers
Orientation
Online Access:Get full text
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Summary:Optical coherence tomography (OCT) and optical coherence microscopy(OCM) have demonstrated the ability to investigate cyto- andmyelo-architecture in the brain. Polarization-sensitive OCT providessensitivity to additional contrast mechanisms, specifically thebirefringence of myelination and, therefore, is advantageous forinvestigating white matter fiber tracts. In this Letter, we developeda polarization-sensitive optical coherence microscope (PS-OCM) with a3.5 mu m axial and 1.3 mu m transverse resolution to investigate fiber organization and orientation at afiner scale than previously demonstrated with PS-OCT. In areconstructed mouse brain section, we showed that at the focal depthsof 20-70 mu m, the PS-OCM reliably identifies theneuronal fibers and quantifies the in-plane orientation.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.41.002213