On the thickness and nanomechanical properties of salivary pellicle formed on tooth enamel

Abstract Objective To determine the thickness and nanomechanical properties of salivary pellicle formed on tooth enamel. Methods In vitro adsorption experiments were conducted by immersing enamel samples in centrifuged saliva for 1 min, and then the nanomechanical properties of the salivary pellicle...

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Bibliographic Details
Published in:Journal of dentistry 2016-12, Vol.55, p.99-104
Main Authors: Zhang, Y.F, Li, D.Y, Yu, J.X, He, H.T
Format: Article
Language:English
Subjects:
Behavior
Biofilms
Dental Caries
Dental Enamel
Dental enamels
Dental Pellicle
Dentistry
Enamel
Hardness
Humans
Indentation
Lubrication
Mathematical models
Measurement techniques
Mechanical model
Microscopy
Nanomechanical property
Nanostructure
Pellicle
Saliva
Salivary pellicle
Salivary Proteins and Peptides
Studies
Teeth
Thickness
Tooth enamel
Topography
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Summary:Abstract Objective To determine the thickness and nanomechanical properties of salivary pellicle formed on tooth enamel. Methods In vitro adsorption experiments were conducted by immersing enamel samples in centrifuged saliva for 1 min, and then the nanomechanical properties of the salivary pellicle/tooth enamel system were measured firstly using nanoindentation based on a continuous stiffness measurement technique. Finally, a model was proposed to obtain the thickness and the intrinsic nanohardness of this biofilm. Results The composite nanohardness of salivary pellicle/tooth enamel system varied with indentation depth. The model can describe the experimental date at both shallow and deep indentation depths very well. The fitted average thickness of salivary pellicle was about 17 nm, which was in good accord with the scanning probe microscopy experimental results. The intrinsic hardness of salivary pellicle and tooth enamel was about 0.52 Gpa and 4.88 Gpa respectively, which was consistent with previous studies. Conclusions It was convenient to extract intrinsic hardness and thickness of salivary pellicle from the indentation curve according to the model. Moreover, this model was applicable to plasticity-dominated behaviour of the soft film/hard substrate system. Clinical significance The research results may be helpful to extend the understanding of our lubricating and anti-caries behaviours of salivary pellicle and to the oral hygiene industry for diagnose oral diseases.
ISSN:0300-5712
1879-176X
DOI:10.1016/j.jdent.2016.10.009